Detection of Herpes Simplex Virus in Clinical Samples by Cell Culture Method

[LANGUAGE= "English"] Purpose: Herpes simplex virus (HSV) infections are a common clinical problem worldwide. Especially HSV disease in the immunocompromised patients can be severe and rapidly progressive that is a significant cause of morbidity and mortality. In this study the aim was to...

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Bibliographic Details
Published in:Journal of Clinical Practice & Research 2009-01, Vol.31 (2), p.99
Main Authors: Gülhan Yağmur, Gökahmetoğlu, Selma, Özbal, Yusuf
Format: Article
Language:Turkish
Subjects:
Cell culture
Cerebrospinal fluid
Herpes viruses
Infections
Methods
Mortality
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Summary:[LANGUAGE= "English"] Purpose: Herpes simplex virus (HSV) infections are a common clinical problem worldwide. Especially HSV disease in the immunocompromised patients can be severe and rapidly progressive that is a significant cause of morbidity and mortality. In this study the aim was to investigate of HSV in clinical specimens by cell culture method.Material and Methods: Various clinical specimens of the 65 patients with suspicious HSV infections treated in Erciyes University Gevher Nesibe Hospitals clinics and applied polyclinics between March 2006 and June 2007 were included in this study. Hep-2 cell culture was used for isolation of HSV.Results: Cytopathic effect (CPE) consistent with HSV was observed in 14 (51.8%) of the 27 swap specimens and 3 (7.8%) of the 38 cerebrospinal fluid specimens which were inoculated to Hep-2 cell culture. Cell culture that was observed CPE was confirmed by DFA (direct fluorescent antibody) method.Conclusion: Cell culture method would be applied to investigate the swab specimens which were taken from patients with HSV infection but CSF specimens is investigated by other methods.
ISSN:2980-2156