The Effects of Conventional Gonadotrophin Therapy on Lymphocyte Subgroups and Its Association with Il-2R Expression In Patients with Idiopathic Hypogonadotrophic Hypogonadism

[LANGUAGE= "English"] Purpose: Although the presence of the interaction between sex steroids and the immune system is known, the mechanism of this interaction, which is very important in understanding the pathogenesis of the autoimmune disorders, is still unclear. In-vivo interaction of te...

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Bibliographic Details
Published in:Journal of Clinical Practice & Research 2007-01, Vol.29 (1), p.1
Main Authors: Tanrıverdi, Fatih, Demirkoparan, Ümit, Bayram, Fahri, Özkul, Yusuf, Munis Dündar, Akalın, Hilal, Patıroğlu, Türkan, Keleştimur, Fahrettin
Format: Article
Language:Turkish
Subjects:
Age
Autoimmune diseases
Cytokines
Flow cytometry
Immune system
Lymphocytes
Pathogenesis
Patients
Testosterone
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Summary:[LANGUAGE= "English"] Purpose: Although the presence of the interaction between sex steroids and the immune system is known, the mechanism of this interaction, which is very important in understanding the pathogenesis of the autoimmune disorders, is still unclear. In-vivo interaction of testosterone and IL-2R expression at lymphocyte level has not yet been investigated. Therefore, in the present study, we report the preliminary results of our ongoing study regarding the effects of conventional gonadotrophin therapy on lymphocyte subgroups and its relation with IL-2R expression in patients with idiopathic hypogonadotrophic hypogonadism (IHH).Materials and Method: Peripheral venous blood samples were obtained from 10 patients with IHH (Mean age 25.6 ± 7.1 years) and 10 age- and sex-matched healthy volunteers (Mean age 27.1 ± 3.9 years). Patients were treated for 12 months (3 days a week) with hCG and hMG. Lymphocyte subtype levels including CD3, CD4, CD8, CD16 and CD19 were analyzed by flow cytometry. Quantitative Real- Time RT-PCR was used to examine the express ion of IL-2R (target gene) and beta-aktin (reference gene) in peripheral mononuclear blood cells (PMBCs) derived from patients and controls.Results: In the patient group, before treatment total lymphocyte count was significantly high and the mean CD4 percentage was significantly low when compared to the results of healthy controls (P
ISSN:2980-2156