Hepatitis type B virus genotypes in chronic Hepatitis B patients (CHBP)

• Main Authors: Hussain, Hiba T. H., Al-Shuwaikh, Arwa Mujahid Abdullah, Ahmed, Abbas. M.
• Format: Conference Proceeding
• Language: English
• Subjects: Females; Hepatitis B; Homogeneity; Immunology; Males; Polymerase chain reaction
• ISSN: 0094-243X; 1551-7616
• DOI: 10.1063/5.0162114

Hepatitis B virus (HBV) complications include liver disease, cirrhosis, and cancer. Every year, approximately one million people die as a result of HBV. Virus sequence homogeneity was used to identify at least ten genotypes (A through J) and multiple subgenotypes. Hepatitis B virus varies in terms of virulence, immunological reactivity, pathogenicity, resistance to cure, and global distribution. The study used the nested PCR technique to identify HBV genotypes among (CHB) patients in Baghdad. One hundred CHB patients (68 males and 32 females, ages(4-70) were included in the study. After six months of infection, 100 patients were diagnosed with chronic HBV using an ELISA test for the presence of Anti-HBc IgG and HBsAg. Nested PCR is used to survey the DNA HBV genotypes in positive samples. Our findings show that genotype D was found in roughly (95%) of patients, with (5%) having mixed genotypes (D+B+C), but genotypes A, E, and F were not found in all patients. In terms of biostatistics, the genotype distribution of females and males differed significantly. The presence of 5 HBV genotypes in both males and females with mixed infection suggests that these people were infected at different points in time and from different sources. An epidemiological evaluation of chronic HBV in Iraqi patients is required to identify aberrant acquisition in these mixed genotypes, which necessitates a clinical evaluation and follow-up of each CHB patient’s status.