PCL-based and Hirudin-containing Composite Nanofibers for Prolonged Anticoagulation Effect

M ore and more concerns about health bring the increasing demand for blood contact tissue engineering alternatives. In this paper, nanoparticles of poly(lactic-co-glycolic acid)/polyethyleneimine mixed with recombinant hirudin(rHNPs) were prepared by a double emulsion solvent volatilization method,...

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Bibliographic Details
Published in:Chemical research in Chinese universities 2023-12, Vol.39 (6), p.1023-1030
Main Authors: Zheng, Zhiwen, Dai, Xin, Li, Xueyang, Du, Chang
Format: Article
Language:English
Subjects:
Analytical Chemistry
Anticoagulants
Blood
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Controlled release
Degradation
Double emulsions
Endothelial cells
Glycolic acid
Hirudin
Inorganic Chemistry
Mechanical properties
Nanofibers
Organic Chemistry
Physical Chemistry
Polycaprolactone
Polyethyleneimine
Tissue engineering
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Summary:M ore and more concerns about health bring the increasing demand for blood contact tissue engineering alternatives. In this paper, nanoparticles of poly(lactic-co-glycolic acid)/polyethyleneimine mixed with recombinant hirudin(rHNPs) were prepared by a double emulsion solvent volatilization method, which were then loaded onto the polycaprolactone(PCL) with polydopamine(PDA) coating to form the composite nanofibers of PCL/PDA/rHNPs. The hydrophilicity and mechanical properties of the composite nanofibers were improved significantly compared with pure PCL. The morphology kept almost unchanged after 30 d of degradation in phosphate buffer saline (PBS). The anticoagulant molecule of hirudin could be gradually released from the composite scaffolds through the degradation of rHNPs in vitro . When the concentration of rHNPs suspension was 5.0 mg/mL, the composite nanofibers could better promote the growth and proliferation of human umbilical vein endothelial cells(HUVECs). The anticoagulant ability of the composite nanofibers was also significantly improved in comparison with that of pure PCL. The design of controlled release anticoagulant materials would alleviate the sudden release of simple fixed hirudin, which could also provide a new idea for the development of novel blood contact materials.
ISSN:1005-9040
2210-3171
DOI:10.1007/s40242-023-3080-7