Hypothalamo–pituitary–adrenal axis activation by administration of cyanamide: a potent inhibitor of aldehyde dehydrogenase

In this report, we investigated the effects of cyanamide (a potent inhibitor of aldehyde dehydrogenase (ALDH: EC 1.2.1.3)) on hypothalamo–pituitary adrenal (HPA)-axis using in situ hybridization histochemistry and radioimmunoassay. Cyanamide administration resulted in a dose-dependent increase in pl...

Full description

Saved in:
Bibliographic Details
Published in:Forensic science international 2000-09, Vol.113 (1), p.397-402
Main Authors: Kinoshita, H, Harbuz, M.S, Jessop, D.S, Finn, D.P, Ameno, S, Ameno, K, Kubota, T, Ijiri, I
Format: Article
Language:English
Subjects:
Alcoholism and acute alcohol poisoning
Aldehyde dehydrogenase
Aldehyde Dehydrogenase - drug effects
Aldehydes
Animals
Arginine Vasopressin - analysis
Arginine Vasopressin - blood
Arginine vasopressin mRNA
Argipressin
Biological and medical sciences
Brain
Brain Chemistry - drug effects
Corticosterone
Corticosterone - analysis
Corticosterone - blood
Corticotrophin releasing factor mRNA
Corticotropin-Releasing Hormone - analysis
Corticotropin-Releasing Hormone - blood
Cyanamide
Cyanamide - adverse effects
Dehydrogenase
Dehydrogenases
Dose-Response Relationship, Drug
Drug Evaluation, Preclinical
Enzymes
Ethanol
Histochemistry
Histocytochemistry
Hybridization
Hypothalamic-pituitary-adrenal axis
Hypothalamo-Hypophyseal System - drug effects
Hypothalamo-Hypophyseal System - enzymology
Hypothalamo–pituitary–adrenal axis
In Situ Hybridization
Inhibitors
Male
Medical sciences
mRNA
Nervous system
Oxidation
Paraventricular nucleus
Pituitary (anterior)
Pituitary-Adrenal System - drug effects
Pituitary-Adrenal System - enzymology
Plasma
Pro-Opiomelanocortin - analysis
Pro-Opiomelanocortin - blood
Proopiomelanocortin
Proopiomelanocortin mRNA
Radioimmunoassay
Rats
Rats, Sprague-Dawley
Toxicology
Vasopressin
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this report, we investigated the effects of cyanamide (a potent inhibitor of aldehyde dehydrogenase (ALDH: EC 1.2.1.3)) on hypothalamo–pituitary adrenal (HPA)-axis using in situ hybridization histochemistry and radioimmunoassay. Cyanamide administration resulted in a dose-dependent increase in plasma corticosterone concentrations, significant increases in not only corticotrophin releasing factor (CRF) mRNA, but also arginine vasopressin (AVP) mRNA in the paraventricular nucleus (PVN) and proopiomelanocortin (POMC) mRNA in the anterior pituitary. These results suggest that cyanamide is able to activate the HPA axis at all levels of the axis.
ISSN:0379-0738
1872-6283
DOI:10.1016/S0379-0738(00)00212-7