Determination of Antibiotic Residues in Boiler Chickens by Liquid Chromatography-Mass Spectrometry

• Published in: Food analytical methods 2023-12, Vol.16 (11-12), p.1618-1626
• Main Authors: Elsayed, Ahmed Sameh, Abusham, Ahmed, Al-Touby, Salem Said Jaroof, Al Rajhi, Waleed Khalid Hilal, Hossain, Mohammad Amzad
• Format: Article
• Language: English
• Subjects: Analytical Chemistry; Antibiotics; Bacteria; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Chickens; Chloramphenicol; Chloromycetin; Chromatography; Ciprofloxacin; Citric acid; Evaporators; Filter paper; Food Science; Gentamicin; Kidneys; Levofloxacin; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Meat; Microbiology; Microorganisms; Oxytetracycline; Poultry; Poultry farming; Residues; Scientific imaging; Sulfanilamide; Superstores; Trichloroacetic acid; Tubes
• ISSN: 1936-9751; 1936-976X
• DOI: 10.1007/s12161-023-02530-4

Antibiotics are those drugs that can be extracted from some microorganisms and they are effective against other organisms, such as bacteria, by either inhibiting the growth of bacteria or by killing them. The present study was conducted to measure the amount of various antibiotics in the local and imported broiler chickens by using liquid chromatography-mass spectrometry (MS/Q-TOF). The samples were collected from Malem Izkat Poultry farm and Al Manara Hypermarket at Al-Dakhiliyah Governorate in Oman. For the extraction of ciprofloxacin and tetracycline groups of antibiotics, each chicken breast meat and kidney sample was taken into the test tubes separately and then added trichloroacetic acid for tetracycline group, then we add citric acid, HNO 3 , and methanol for ciprofloxcine groups. The supernatant was filtered through 0.45-μm nylon filter paper and pre-concentrated by using a rotary evaporator. As standard antibiotics, gentamicin sulfate, sulfanilamide, oxytetracycline, chloramphenicol, and levofloxacin were used to detect and quantify in the kidney and breast meat samples. The results showed that all antibiotics detected in the samples are within the permissible limit. However, for imported chicken breast meat, gentamicin and levofloxacin were absent from the five antibiotics. In the local breast meat, the highest amount was gentamicin followed by sulfanilamide > oxytetracycline > chloramphenicol > levofloxacin, respectively. Regarding imported chicken, the highest amount was gentamicin followed by oxytetracycline and levofloxacin, respectively. In conclusion, the low concentration of antibiotics residues detected in chicken meat and kidney collected from all the samples does not create a significant health problem for the consumers.