Genome analysis of clinical genotype Vibrio vulnificus isolated from seafood in Mangaluru Coast, India provides insights into its pathogenicity

• Published in: The Veterinary quarterly 2023-12, Vol.43 (1), p.1-17
• Main Authors: Prithvisagar, Kattapuni Suresh, Gollapalli, Pavan, D'Souza, Caroline, Rai, Praveen, Karunasagar, Iddya, Karunasagar, Indrani, Kumar, Ballamoole Krishna
• Format: Article
• Language: English
• Subjects: Antibiotics; Antimicrobial agents; Antimicrobial peptides; Antimicrobial resistance; Bacteria; Biofilms; Bioinformatics; Chemical analysis; Deoxyribonucleic acid; DNA; Estuarine environments; Gene sequencing; Genes; Genomes; Genomic analysis; Genotypes; India; Multilocus sequence typing; Nucleotide sequence; Opportunist infection; Pathogenesis; Pathogenicity; Pathogens; Phylogeny; Proteins; Public health; Quorum sensing; Seafood; Seawater; Sequence analysis; Vibrio vulnificus; Virulence; Water analysis; whole genome sequence
• ISSN: 0165-2176; 1875-5941
• DOI: 10.1080/01652176.2023.2240389

Vibrio vulnificus an opportunistic human pathogen native to marine/estuarine environment, is one of the leading causes of death due to seafood consumption and exposure of wounds to seawater worldwide. The present study involves the whole genome sequence analysis of an environmental strain of V. vulnificus (clinical genotype) isolated from seafood along the Mangaluru coast of India. The sequenced genome data was subjected to in-silico analysis of phylogeny, virulence genes, antimicrobial resistance determinants, and secretary proteins using suitable bioinformatics tools. The sequenced isolate had an overall genome length of 4.8 Mb and GC content of 46% with 4400 coding DNA sequences. The sequenced strain belongs to a new sequence type (Multilocus sequence typing) and was also found to branch with a phylogenetic lineage that groups the most infectious strains of V. vulnificus. The seafood isolate had complete genes involved in conferring serum resistance yet showed limited serum resistance. The study identified several genes against the antibiotics that are commonly used in their treatment, highlighting the need for alternative treatments. Also, the secretory protein analysis revealed genes associated with major pathways like ABC transporters, two-component systems, quorum sensing, biofilm formation, cationic antimicrobial peptide (CAMP) resistance, and others that play a critical role in the pathogenesis of the V. vulnificus. To the best of our knowledge, this is the first report of a detailed analysis of the genomic information of a V. vulnificus isolated from the Indian subcontinent and provides evidence that raises public health concerns about the safety of seafood.