Stabilization of DNA-encapsulating Droplets through Negative Charge at the Droplet Interface

It was found that stable cell-sized droplets entrapping DNA molecules are spontaneously generated through micro segregation. To clarify the mechanism, we performed measurements of the electrification by adapting a polymer solution with polyethylene glycol/dextran. We determined the Donnan potential...

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Bibliographic Details
Published in:Chemistry letters 2023-10, Vol.52 (10), p.794-797
Main Authors: Shono, Mayu, Fujita, Fumika, Yoshikawa, Kenichi, Shioi, Akihisa
Format: Article
Language:English
Subjects:
Dextrans
Droplets
Polyethylene glycol
Zeta potential
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Summary:It was found that stable cell-sized droplets entrapping DNA molecules are spontaneously generated through micro segregation. To clarify the mechanism, we performed measurements of the electrification by adapting a polymer solution with polyethylene glycol/dextran. We determined the Donnan potential for macroscopic phase segregation in both the presence and absence of DNA, together with the measurements of Zeta potentials of the microphase solution with number of small droplets. We confirmed that the droplets entrapping DNA are negatively charged.
ISSN:0366-7022
1348-0715
DOI:10.1246/cl.230294