Transcriptome analysis of Iris japonica var. variegata for chlorophyll and photosynthesis-related pathways involved in white–green striped leaves

As a well-known garden plant, many beautiful iris flower phenotypes and leaf variegation have been created. Iris japonica var. variegata is a variant of I. japonica that has a white–green striped leaf color genotype. It has high horticultural value, but its underlying molecular mechanisms remain unc...

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Bibliographic Details
Published in:Acta physiologiae plantarum 2024, Vol.46 (1), Article 12
Main Authors: Yu, Xiaofang, Yue, Linjie, He, Qingling, Li, Shurui, Zheng, Yunhao, Wang, Zhiwen, Wang, Heting, Liu, Rouzhi, Huang, Xin, Xiao, Xue, Yang, Lijuan, Lei, Ting
Format: Article
Language:English
Subjects:
Agriculture
Biomedical and Life Sciences
Chlorophyll
Chloroplasts
Down-regulation
Gene expression
Genes
Genotypes
Leaves
Life Sciences
Molecular modelling
Original Article
Phenotypes
Photosynthesis
Plant Anatomy/Development
Plant Biochemistry
Plant breeding
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plant tissues
Porphyrins
Transcriptomes
Variegation
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Summary:As a well-known garden plant, many beautiful iris flower phenotypes and leaf variegation have been created. Iris japonica var. variegata is a variant of I. japonica that has a white–green striped leaf color genotype. It has high horticultural value, but its underlying molecular mechanisms remain unclear. Here, we analyzed the physiological features and ultrastructural observation of white and green tissues. In our study, the results showed that the phenotype was related to the abnormal chloroplast morphology and deficiencies in chlorophyll. To understand the mechanisms for these observations, we used Illumina sequencing technology to identify the transcriptome and profile differentially expressed genes (DEGs). We identified 1327 DEGs in white leaf tissues compared with green leaf tissues, including 342 upregulated genes and 985 downregulated DEGs. The downregulated DEGs included porphyrin and chlorophyll metabolism, chloroplast development, and photosynthetic pathways. Changes in the expression of genes ( CYSG and GLK ) involved in these pathways could be responsible for the phenotype of I. japonica var. variegata . To our knowledge, this is the first time that evidence has been provided to better understand the primary cause of chloroplast structure in white–green striped leaves for iris and will be valuable for the iris breeding industry.
ISSN:0137-5881
1861-1664
DOI:10.1007/s11738-023-03638-0