Isolation of aquaporin-encoding gene from cassava (Manihot esculenta Crantz) genotype “Ubi Kuning” induced by drought stress

• Main Authors: Hartati, N. Sri, Wahyuni, Pandegan, Risang, Semiarti, Endang, Sudarmonowati, Enny
• Format: Conference Proceeding
• Language: English
• Subjects: Amino acids; Aquaporins; Biofuels; Cassava; Drought; Gene sequencing; Homology; Raw materials
• ISSN: 0094-243X; 1551-7616
• DOI: 10.1063/5.0183424

Cassava is a drought-resistance commodity, that is highly potential to be developed as raw material for food, feed, and bioethanol industries. Production of cassava can be enhanced through biotechnology within the crisis of water and climate change challenges. The objectives of this research are to isolate the gene encoding aquaporin from Manihot esculenta genotype Ubi Kuning hereafter called the MeAQP gene, which is induced by drought stress, and also to evaluate the homology among MeAQP sequences. MeAQP isolation was initiated with MeAQP mRNA isolation from a leaf that has been induced by dryness for 26 days, and then followed by first-strand cDNA synthesis, optimizing PCR condition for RT-PCR with specific gene primers, gene sequencing, and finally, homology analysis using Chromas, BLAST, BioEdit, and CLUSTAL Omega. MeAQP plays a role to keep cell turgidity pressure and water available. MeAQP of Ubi Kuning resistance to drought treatment is homolog with the aquaporin gene from GeneBank has a high identity percentage among gene sequences (>70%), and has a conserved domain such as NPA amino acid (Asn-Pro-Aln)-an amphipathic channel that indicates drought resistance capability.