Effect of preparation method on moringa leaf extract (Moringa oleifera Lamk) nanoemulsion and propolis

• Main Authors: Fauziah, Amalia Nur, Sondari, Dewi, Hanifah, Arzqa Sabila, Ermawar, Riksfardini Annisa, Hermiati, Euis, Pramasari, Dwi Ajias, Mahani, Septiyanti, Melati, Dewi, Rizna Triana, Sumarlin, La Ode, Ningrum, Riska Surya, Hasan, Fahmi
• Format: Conference Proceeding
• Language: English
• Subjects: Antioxidants; Liquid chromatography; Mass spectrometry; Nanoemulsions; Propolis; Reagents; Stability analysis
• ISSN: 0094-243X; 1551-7616
• DOI: 10.1063/5.0184781

This study aims to make the optimum formulation of nanoemulsions from Moringa leaf extract (Moringa oleifera Lamk) and propolis using treatment ultrasonication and ultra turrax with variations in time and speed. This nanoemulsion is a finely dispersed oil-in-water (o/w) emulsion system that has droplet sizes in the range of 50–1000 nm. The water phase consists of aquadest, Moringa leaf extract, propolis, and tween 80 as a surfactant, while the oil phases span 80 and white oil. Nanoemulsions were made with a variation of water-based 87.5%-97.5%. The characteristics of nanoemulsion were characterized by Particle Size Analyzer (PSA), stability, and microscope digital. While the extract of moringa leaf and propolis was characterized by antioxidant content and Liquid Chromatography Mass Spectrometry (LCMS). According to the antioxidant test using DPPH reagent, propolis and Moringa leaf extract both had IC50 values for antioxidants of 78,227 and 95,996 ppm, respectively. While kaempferol and quercetin with molecular weights of 595, 449, and 465 were found in the LCMS analysis. This research found that the smallest size of nanoemulsion was 196.03 nm with a formulation that uses base water of 97.5%.