Antioxidant Gallic acid protects clotting factor proteins against Hydrogen peroxide induced oxidative injury

Hydrogen peroxide (H2O2) is reactive oxygen species produced by cellular metabolism in human body. This in vitro study was designed to measure oxidative injury induced in clotting factors and plasma proteins by H2O2 and determined the capability of Gallic acid to protect clotting factors and plasma...

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Bibliographic Details
Published in:Research journal of pharmacy and technology 2023-07, Vol.16 (7), p.3463-3467
Main Author: Q Jaradat, Ahmad
Format: Article
Language:English
Subjects:
Acids
Antioxidants
Blood & organ donations
Enzymes
Hydrogen peroxide
Oxidative stress
Plasma
Proteins
Reactive oxygen species
Statistical analysis
Variance analysis
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Summary:Hydrogen peroxide (H2O2) is reactive oxygen species produced by cellular metabolism in human body. This in vitro study was designed to measure oxidative injury induced in clotting factors and plasma proteins by H2O2 and determined the capability of Gallic acid to protect clotting factors and plasma proteins against H2O2-induced oxidative injury. The capability of Gallic acid to resist H2O2-induced oxidation was estimated by measuring the prothrombin time (PT), activated partial thromboplastin time (aPTT), protein carbonyl and malondialdehyde formation in experimental groups. Incubation of clotting factors and plasma proteins with 0.5% H2O2 for 30 minutes resulted in significantly increase PT, aPTT, and significantly rise levels of malondialdehyde and carbonyl groups formation. Gallic acid significantly decreased PT and aPTT and reduced carbonyl group and malondialdehyde formation in plasma proteins. Therefore, the antioxidant Gallic acid protects clotting factors and plasma proteins from oxidative injury induced by H2O2.
ISSN:0974-3618
0974-360X
0974-306X
DOI:10.52711/0974-360X.2023.00572