Environmental DNA-based quantification of Edwardsiella bacteria and fish-derived materials in rearing water of infected ayu Plecoglossus altivelis and red sea bream Pagrus major

The bacterial genus Edwardsiella includes important fish pathogens that cause serious economic damage to commercial fisheries. To understand the infection cycle of bacteria of this genus, we carried out experimental infections of ayu Plecoglossus altivelis with Edwardsiella ictaluri and of red sea b...

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Bibliographic Details
Published in:Fisheries science 2024, Vol.90 (1), p.93-103
Main Authors: Takeuchi, Hisato, Kawakami, Hidemasa, Mano, Nobuhiro, Yamanaka, Hiroki, Shimizu, Sonoko
Format: Article
Language:English
Subjects:
Bacteria
Biomedical and Life Sciences
Bream
Cells
Edwardsiella
Environmental DNA
Fish
Fish & Wildlife Biology & Management
Fisheries
Food Science
Freshwater & Marine Ecology
Individual rearing
Life Sciences
Marine fishes
Original Article
Pagrus major
Pathogens
Plecoglossus altivelis
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Summary:The bacterial genus Edwardsiella includes important fish pathogens that cause serious economic damage to commercial fisheries. To understand the infection cycle of bacteria of this genus, we carried out experimental infections of ayu Plecoglossus altivelis with Edwardsiella ictaluri and of red sea bream Pagrus major with Edwardsiella anguillarum , and quantified the bacteria and fish-derived materials in their rearing water by measuring environmental DNA (eDNA). The eDNA concentration of both Edwardsiella bacteria and fish in rearing water of infected fish trended upward in all experiments, and the fish eDNA concentrations of infected fish were significantly higher than those of non-infected control fish. There were significant positive correlations between eDNA concentrations of Edwardsiella bacteria and fish. Additionally, in the experiment with red sea bream, the number of skin epithelial-like cells of red sea bream in the rearing water of infected fish was significantly higher than in that of control fish, and the number of cells showed a high positive correlation with the eDNA concentration of red sea bream. These results suggest that release amounts of both Edwardsiella bacteria and fish-derived materials from infected fish increase with the progression of infection.
ISSN:0919-9268
1444-2906
DOI:10.1007/s12562-023-01724-3