Analysis of the Functional State of Astroglia in the Hippocampus of Rats Genetically Prone to Audiogenic Seizures

It was shown that abnormalities in astrocyte functions can cause alterations in neuronal excitability and promote the development of epilepsy, however, this question claims further investigation. The aim of the present work was to analyze the functional state of astrocytes in the hippocampus of Krus...

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Published in:Journal of evolutionary biochemistry and physiology 2023-11, Vol.59 (6), p.1924-1934
Main Authors: Grigorieva, Yu. S., Naumova, A. A., Nikolaeva, S. D., Glazova, M. V.
Format: Article
Language:English
Subjects:
Animal Physiology
Aquaporin 4
Astrocytes
Auditory stimuli
Biochemistry
Biomedical and Life Sciences
Convulsions & seizures
Epilepsy
Evolutionary Biology
Excitability
Experimental Papers
Glial fibrillary acidic protein
Gliosis
Glucose metabolism
Glutamate-ammonia ligase
Glycolysis
Hippocampus
Life Sciences
Metabolism
Neuronal-glial interactions
Osteonectin
Protein transport
Seizures
Synapses
Synaptogenesis
Zebrin II
γ-Aminobutyric acid
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Summary:It was shown that abnormalities in astrocyte functions can cause alterations in neuronal excitability and promote the development of epilepsy, however, this question claims further investigation. The aim of the present work was to analyze the functional state of astrocytes in the hippocampus of Krushinsky–Molodkina (KM) rats genetically predisposed to the reflex audiogenic seizures. Adult naïve animals with fully-established audiogenic sensitivity were recruited in our experiments. Rats of parental Wistar strain were used as a control. Obtained results revealed unchanged protein expression of such astocytic markers as GFAP, ALDH1L1, and NFIA suggesting the absence of the reactive astrogliosis in the hippocampus of KM rats. SPARC and aquaporin 4 expression also did not differ from the control indicating no violations in astroglia regulation of synaptogenesis and water transport. Analysis of glial proteins responsible for reuptake and metabolism of neurotransmitters revealed normal expression of GABA transporter GAT-3 and glutamine synthetase, while glutamate transporters EAAT1 and 2 were significantly elevated pointing on enhanced activity of glutamate removal from synapses. In addition, decreased expression of the key glycolytic enzyme aldolase C, probably, indicated insufficient activity of glucose metabolism in astrocytes. Thus, obtained data pointed on genetically determined alterations of astroglia functions in the hippocampus of rats with inherited reflex epilepsy.
ISSN:0022-0930
1608-3202
DOI:10.1134/S0022093023060030