Phytochemical Screening, Chromatographic and Quantitative Study of Phenols and Flavonoids in Leaves of Oroxylum indicum and Pongamia pinnata

• Published in: Research journal of pharmacy and technology 2023-06, Vol.16 (6), p.2604-2608
• Main Authors: Chandewar, Anil V, Kochar, Nitin I, Shrirao, Abhijit V, Karpe, Sujit T
• Format: Article
• Language: English
• Subjects: Amino acids; Carbohydrates; Chromatography; Dietary minerals; Fatty acids; Flavonoids; Leaves; Phenols; Phytochemicals; Proteins; Quantitative analysis; Reagents; Seeds; Solvents
• ISSN: 0974-3618; 0974-360X; 0974-306X
• DOI: 10.52711/0974-360X.2023.00427

Number of secondary compounds is produced by plants as natural antioxidants. The current study's goal was to identify the phytochemical components, thin-layer chromatographic profile, total phenol, and total flavonoid content in the leaves of Pongamia pinnata and Oroxylum indicum (O. indicum) (P. pinnata). The well-known test protocol described in the literature was used to determine the qualitative analysis of different phytochemical constituents, thin-layer chromatography, and quantitative analysis of total phenolics and flavonoids. By using the Folins Ciocalteau reagent method and the aluminium chloride method, respectively, phenolic and flavonoid quantities were analysed quantitatively. Both plants contained glycosides, flavonoids, diterpenes, phenol, proteins, saponins, and carbohydrates, according to a phytochemical analysis. Studies using thin-layer chromatography and various solvent systems revealed homogeneous spots with various Rf values. According to the findings of the current study, O. indicum and P. pinnata crude extracts are a rich source of secondary phytoconstituents that have significant antioxidant potential. The pharmaceutical industries, pharmacologists, and phytochemists will all benefit from the study's findings.