Effect of in vitro simulated digestion on the biological activities of purified phenolic compounds from Moringa oleifera leaves

Summary The biological activities of Moringa oleifera leaf polyphenols were tested after in vitro gastrointestinal (GI) digestion and colonic fermentation (CF). Following in vitro GI digestion and CF, total phenolic compounds (TPC) were reduced by 30% and 38.82%, respectively. Moringa polyphenol ext...

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Bibliographic Details
Published in:International journal of food science & technology 2024-02, Vol.59 (2), p.985-994
Main Authors: Hamed, Yahya S., Akhtar, Hafiz Muhammad Saleem, Rayan, Ahmed M., Zeng, Xiaoxiong
Format: Article
Language:English
Subjects:
Cell proliferation
Cytotoxicity
Digestion
Digestive system
Energy harvesting
Energy metabolism
Fermentation
Gastrointestinal tract
Glucose metabolism
Glucosidase
Gut microbiota
inhibitory activity
Intestinal microflora
Moringa oleifera
Moringa polyphenols extract
Phenolic compounds
Phenols
Polyphenols
Risk reduction
α-Amylase
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Summary:Summary The biological activities of Moringa oleifera leaf polyphenols were tested after in vitro gastrointestinal (GI) digestion and colonic fermentation (CF). Following in vitro GI digestion and CF, total phenolic compounds (TPC) were reduced by 30% and 38.82%, respectively. Moringa polyphenol extract (MPPE) substantially reduced pancreatic lipase and α‐glucosidase inhibition by 5.63% and 14.20% after GI digestion and 27.41% and 34.01% after CF. In contrast, inhibitory activity against α‐amylase was eliminated with GI digestion and CF. Slight decreases in both antioxidant activity assays (DPPH and ABTS) after GI digestion and CF were observed. Cytotoxic activity against HeLa and HepG2 cancer cells was decreased after GI digestion and CF while anti‐proliferation activity against HepG2 cells was improved after CF. MPPE significantly affected the abundance of gut microbiota groups, accordingly, could improve glucose metabolism, decline the energy harvest and subsequently reduce the risk of obesity. Effect of sequential digestion on isolated polyphenols from M. oleifera was studied. Total phenols and inhibitory activity against metabolic enzymes were decreased. Anti‐proliferation activity against HepG2 cancer cells improved after CF. MPPE significantly affected the abundance of gut microbiota groups. As functional food, supplementation with moringa could improve human health.
ISSN:0950-5423
1365-2621
DOI:10.1111/ijfs.16858