Assessing the production of galactooligosaccharides in batch and continuous mode by using β‐galactosidase immobilised on mesoporous silicon dioxide nanoparticles

Summary The present study evaluated the production of galactooligosaccharides (GOSs) from lactose in both batch and continuous modes using nano‐immobilised β‐galactosidase. Mesoporous silicon dioxide nanoparticles were used to immobilise β‐galactosidase to enhance the stability and production of GOS...

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Bibliographic Details
Published in:International journal of food science & technology 2024-02, Vol.59 (2), p.939-949
Main Authors: C. T., Manoj Kumar, S., Supreetha, M. H., Sathish Kumar, Rao, Priyanka Singh, K., Jayaraj Rao
Format: Article
Language:English
Subjects:
Electron microscopes
Enzymatic activity
Enzyme activity
Enzymes
Fourier transforms
Galactooligosaccharides
Galactosidase
immobilisation
Immobilization
Infrared analysis
Lactose
Nanoparticles
packed bed reactor
Packed beds
Scanning electron microscopy
Silica
Silicon
Silicon dioxide
silicon dioxide nanoparticles
Stability analysis
Substrate preferences
Substrates
β‐galactosidase
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Summary:Summary The present study evaluated the production of galactooligosaccharides (GOSs) from lactose in both batch and continuous modes using nano‐immobilised β‐galactosidase. Mesoporous silicon dioxide nanoparticles were used to immobilise β‐galactosidase to enhance the stability and production of GOSs. The change in enzyme‐characteristics was assessed using Fourier‐transform infrared and scanning electron microscope analysis. The nano‐immobilisation increased the enzyme's substrate affinity, but decreased its hydrolytic activity. A significant increase (P 
ISSN:0950-5423
1365-2621
DOI:10.1111/ijfs.16854