P183 Urea induced ros accelerate renal failure progression

• Published in: Archives of disease in childhood 2017-06, Vol.102 (Suppl 2), p.A104-A105
• Main Authors: D’Apolito, Maria, Colia, Anna Laura, Caggianello, Roberta, Pettoello-Mantovani, Massimo, Brownlee, Michael, Maffione, Angela Bruna, Giardino, Ida
• Format: Article
• Language: English
• Subjects: Adipocytes; End-stage renal disease; Endothelial cells; Kidney diseases; Kidneys; Mitochondria; Monocyte chemoattractant protein 1; Morbidity; Pediatrics; Renal failure; Renal function; Toxicity; Toxins; Urea
• ISSN: 0003-9888; 1468-2044
• DOI: 10.1136/archdischild-2017-313273.271

Background and aimsChronic kidney disease (CKD) in children is an irreversible process that may lead to end-stage renal disease (ESRD). Uremic toxins have been suggested to promote progression of chronic renal failure. Urea has been long considered to have negligible toxicity in patients with CKD. In contrast, a consistent number of in vitro and in vivo studies have recently shown a direct toxicity of urea. Here we investigate the hypothesis that concentrations of urea associated with CKD and increased ROS production in adipocytes and endothelial cells might also increase ROS production directly in human podocytes, causing abnormalities which promote the progression of kidney damagesMethodsHuman conditionally immortalised podocytes were cultured in the presence or absence of 20 mM urea.ResultsUrea at concentrations usually seen in CKD, induce mitochondrial ROS production in cultured human podocytes, leading to cellular dysfunctions typically seen in the CKD progression. Urea induced ROS decrease nephrin and podocine expression (markers of renal function), increase p65 and MCP-1 expression (markers of inflammation), and increase the intracellular lipid synthesis, as shown by the increased expression and activity of the transcription factor SERBP1. Normalisation of mitochondrial ROS production prevents each of these effects of urea. In uremic mice, treatment with MnTBAP prevents the progression of renal failure.ConclusionsTaken together, these data suggest that urea itself contributes to the progression of renal failure Since urea’s effects are caused by its capacity to induce mitochondrial ROS production, reduction in the high morbidity and mortality caused by ESRD may be achieved by novel therapeutics that directly target urea-induced ROS.