Antimicrobial potency of catfish (Clarias gariepinus) mucus

Catfish mucus is secreted by epidermal tissue, which functions as a defense against parasites. Fish mucus contains various antimicrobial substances. The purpose of this study was to determine the antibacterial and antifungal activities of catfish (Clarias gariepinus) mucilage extracts on Methicillin...

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Bibliographic Details
Main Authors: Widiyani, Tetri, Astuti, Ririn D., Azizah, Ranita R. N., Listyawati, Shanti, Purwoko, Tjahjadi, Budiharjo, Agung, Setyaningsih, Ratna
Format: Conference Proceeding
Language:English
Subjects:
Acetic acid
Biological activity
Catfish
Electrophoresis
Fungicides
Lysozyme
Microorganisms
Mucus
Proteins
Pseudomonas aeruginosa
Staphylococcus infections
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Summary:Catfish mucus is secreted by epidermal tissue, which functions as a defense against parasites. Fish mucus contains various antimicrobial substances. The purpose of this study was to determine the antibacterial and antifungal activities of catfish (Clarias gariepinus) mucilage extracts on Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa ATCC 27853, and Aspergillus flavus as well determine the protein profile. The catfish mucus sample was extracted in a glacial acetic acid solvent. Antimicrobial testing was performed in vitro by using the disc diffusion method to determine inhibitory activity on microbials growth. The protein profile contained in the mucus was electrophored by using the SDS-PAGE method. The antimicrobial test showed that catfish mucus performs an inhibition zone against MRSA and P. aeruginosa ATCC 27853 but not against A. flavus. Aspergilus flavus was not inhibited by catfish mucus extract because fungi are known to have a relatively high survival rate. Electrophoresis results in 4 protein bands with molecular weight (MW) respectively 117.56; 58.82; 25.37; and 15.47 kDa. Protein band 15.47 kDa is rather similar to lysozyme, a protein that is capable of eliciting microbial activity. Nevertheless, it indicated the presence of low-level protein contents. Therefore, the antimicrobial activities are relatively low. It needs further study to explore the potential of catfish mucus as an antimicrobial deeply.
ISSN:0094-243X
1551-7616
DOI:10.1063/5.0183190