The Biotechnological Potential of Methylotrophic Yeast Komagataella phaffii (Pichia pastoris) for Assembly of Bacteriophage MS2 Virus-Like Particles

Successful assembly of bacteriophage MS2 virus-like particles (VLPs) from a secreted recombinant capsid protein in the culture medium of the Komagataella phaffii methylotrophic yeast has been shown. The yield of VLPs was 5 g/L and reached 30% of the total protein of the culture liquid supernatant. T...

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Bibliographic Details
Published in:Applied biochemistry and microbiology 2023-12, Vol.59 (9), p.1132-1139
Main Authors: Borschevskaya, L. N., Gordeeva, T. L., Pichkur, E. B., Samygina, V. R., Sineoky, S. P.
Format: Article
Language:English
Subjects:
Assembly
Biochemistry
Biology
Biomedical and Life Sciences
Biotechnology
Capsid protein
Gene Engineering
Komagataella phaffii
Life Sciences
Medical Microbiology
Microbiology
Phages
Producers
Proteins
Selection
Transmission electron microscopy
Virus-like particles
Viruses
Yeast
Yeasts
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Summary:Successful assembly of bacteriophage MS2 virus-like particles (VLPs) from a secreted recombinant capsid protein in the culture medium of the Komagataella phaffii methylotrophic yeast has been shown. The yield of VLPs was 5 g/L and reached 30% of the total protein of the culture liquid supernatant. The VLPs were well-ordered icosahedral structures with a diameter of 26‒28 nm, which was confirmed by transmission electron microscopy. A simple method for the isolation and purification of the VLPs was proposed, which ensures the purity of the target protein structures of more than 90%. The results we obtained can be used for development of a technology for the large-scale production of recombinant vaccines based on bacteriophage MS2 VLPs.
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683823090028