Microsatellites reveal high levels of genetic admixture in the natural populations of Laurus azorica, Lauraceae

• Published in: Plant systematics and evolution 2024-02, Vol.310 (1), p.1-17, Article 4
• Main Authors: Rego, Rúben, Vieira, Ângela F., Silva, Luís, Pires Bento da Silva Elias, Rui Miguel, Silva, Carlos, Resendes, Roberto, Tavares de Moura, Mónica Maria
• Format: Article
• Language: English
• Subjects: Alleles; Amplification; Archipelagoes; Azores; Bayesian analysis; Biomedical and Life Sciences; Conservation; Conservation Strategies; Gene flow; Genetic Diversity; Genetics; Hybridization; Islands; Lauraceae; Laurus; Laurus Azorica; Life Sciences; Microsatellites; Natural populations; Plant Anatomy/Development; Plant Ecology; Plant Sciences; Plant Systematics/Taxonomy/Biogeography; Population dynamics; Population genetics; Population Structure; Populations; Private Alleles; Refugia; Taxonomy; Translocation
• ISSN: 0378-2697; 1615-6110; 2199-6881
• DOI: 10.1007/s00606-023-01888-6

Laurus (Lauraceae) species are currently restricted to isolated refugia in the southern Black Sea, Mediterranean Basin, southern Morocco, and Macaronesian archipelagos. One to three species of Laurus has been recognized: the Azorean endemic Laurus azorica, L. nobilis from the Mediterranean, and L. novocanariensis from Madeira and the Canary Islands. This study aims to determine the population structure, genetic diversity, and associated patterns of gene flow within and between Azorean populations, using eight existing SSR markers. We also included plant material from L. nobilis populations found in the Azores, for comparison. Amplification was performed in 212 samples of L. azorica and in 30 samples of L. nobilis. For L. azorica, 78 alleles were amplified (average 9.75 alleles per loci). Bayesian analysis with STRUCTURE unveiled five genetic groups for the Azorean accessions, with high level of genetic admixture. Genetic diversity was generally high, with moderate levels of genetic differentiation among L. azorica populations. Moreover, high gene flow levels, likely mediated by past human translocations and naturally, by birds, might have contributed to the high level of genetic admixture observed possibly reflecting hybridization events between L. nobilis and L. azorica. Conservation measures should be applied to some populations of São Miguel, Santa Maria, and Flores Islands, based on the number of private alleles, and further, ad hoc translocation events should be avoided. Conservation in situ and the preservation of laurel forest remains are recommended. The study of this species’ taxonomy, genetics, and population dynamics in the Macaronesian region should be continued.