Extracellular synthesis of silver nanoparticle using yeast extracts: antibacterial and seed priming applications

• Published in: Applied microbiology and biotechnology 2024-12, Vol.108 (1), p.150, Article 150
• Main Authors: Kim, Dae-Young, Kim, Min, Sung, Jung-Suk, Koduru, Janardhan Reddy, Nile, Shivraj Hariram, Syed, Asad, Bahkali, Ali H., Seth, Chandra Shekhar, Ghodake, Gajanan Sampatrao
• Format: Article
• Language: English
• Subjects: Antibacterial activity; Antimicrobial agents; Antimicrobial resistance; Bacteria; Biomedical and Life Sciences; Biotechnological Products and Process Engineering; Biotechnology; Contamination; Functional groups; Germination; Gram-negative bacteria; Life Sciences; Microbial Genetics and Genomics; Microbiology; Microorganisms; Multidrug resistance; Nanoparticles; Priming; Production methods; Renewable resources; Seed germination; Seed-borne diseases; Seedlings; Silver; Sorghum; Synthesis; Yeast; Yeasts
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-023-12920-7

The evolution and rapid spread of multidrug-resistant (MDR) bacterial pathogens have become a major concern for human health and demand the development of alternative antimicrobial agents to combat this emergent threat. Conventional intracellular methods for producing metal nanoparticles (NPs) using whole-cell microorganisms have limitations, including binding of NPs to cellular components, potential product loss, and environmental contamination. In contrast, this study introduces a green, extracellular, and sustainable methodology for the bio-materialization of silver NPs (AgNPs) using renewable resource cell-free yeast extract. These extracts serve as a sustainable, biogenic route for both reducing the metal precursor and stabilizing the surface of AgNPs. This method offers several advantages such as cost-effectiveness, environment-friendliness, ease of synthesis, and scalability. HR-TEM imaging of the biosynthesized AgNPs revealed an isotropic growth route, resulting in an average size of about ~ 18 nm and shapes ranging from spherical to oval. Further characterization by FTIR and XPS results revealed various functional groups, including carboxyl, hydroxyl, and amide contribute to enhanced colloidal stability. AgNPs exhibited potent antibacterial activity against tested MDR strains, showing particularly high efficacy against Gram-negative bacteria. These findings suggest their potential role in developing alternative treatments to address the growing threat of antimicrobial resistance. Additionally, seed priming experiments demonstrated that pre-sowing treatment with AgNPs improves both the germination rate and survival of Sorghum jowar and Zea mays seedlings. Key points •Yeast extract enables efficient, cost-effective, and eco-friendly AgNP synthesis. •Biosynthesized AgNPs showed strong antibacterial activity against MDR bacteria. •AgNPs boost seed germination and protect against seed-borne diseases. Graphical Abstract