Preparation, characterization and in vitro biological study of silk fiber/methylcellulose composite for bone tissue engineering applications

• Published in: Polymer bulletin (Berlin, Germany) Germany), 2019-06, Vol.76 (6), p.2777-2800
• Main Authors: Narayanan, Valarmathi, Sumathi, Shanmugam
• Format: Article
• Language: English
• Subjects: Antioxidants; Biocompatibility; Biological activity; Biomedical materials; Biomimetics; Body fluids; Bones; Cellulose; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Complex Fluids and Microfluidics; Composite materials; E coli; Emission analysis; Fourier transforms; Gram-positive bacteria; Hydrogen peroxide; In vitro methods and tests; Inductively coupled plasma; Infrared spectroscopy; Leaching; Lithium; Mechanical properties; Optical emission spectroscopy; Organic Chemistry; Original Paper; Physical Chemistry; Polymer Sciences; Polymers; Silk; Soft and Granular Matter; Surface stability; Tensile strength; Thermal stability; Tissue engineering; X ray powder diffraction; X-ray diffraction
• ISSN: 0170-0839; 1436-2449
• DOI: 10.1007/s00289-018-2518-4

In the present work, silk fiber (SF) and methylcellulose (MC) composites were fabricated by solvent casting method and characterized in detail. The interactions between SF/MC composites were studied in detail by Fourier transform infrared (FT-IR) spectroscopy and powder X-ray diffraction (XRD). The surface morphology and thermal stability were studied. Viscosity, thickness, folding endurance, tensile strength and antioxidant activity were analyzed for different ratios of SF/MC composite. Antimicrobial activity, in vitro biomimetic mineralization, hemocompatibility and cell viability of the SF/MC composite were studied. The deposition of calcium and phosphorus ions from simulated body fluid (SBF) onto SF/MC composite surface was evidenced from XRD, FT-IR and SEM–EDS. Inductively coupled plasma-optical emission spectrometry analysis (ICP-OES) was utilized to analyze leaching of Ca and P ions from the SBF. Hemolytic assay proves that the composites were compatible with blood and hemolytic ratio is found to be less than 5%. The MTT assay test against MG-63 suggests that the SF/MC composites are promising biomaterials for bone tissue engineering applications.