Glycosylated Gadolinium as Potential Metabolic Contrast Agent vs Gd-DTPA for Metabolism of Tumor Tissue in Magnetic Resonance Imaging

• Published in: Applied magnetic resonance 2016-04, Vol.47 (4), p.375-385
• Main Authors: Heydarnezhadi, Sara, Riahi Alam, Nader, Haghgoo, Soheila, Ghanaati, Hossein, Khoobi, Mehdi, Gorji, Ensiyeh, Rafiei, Behrooz, Nikfari, Banafsheh, Amirrashedi, Mahsa
• Format: Article
• Language: English
• Subjects: Atoms and Molecules in Strong Fields; Bioaccumulation; Cancer; Contrast agents; Fourier transforms; Gadolinium; Glucose; Hemodialysis; High resolution electron microscopy; Image contrast; In vivo methods and tests; Inductively coupled plasma; Infrared spectroscopy; Laser Matter Interaction; Magnetic resonance imaging; Medical imaging; Medical prognosis; Metabolism; Nanoparticles; Organic Chemistry; Photon correlation spectroscopy; Physical Chemistry; Physics; Physics and Astronomy; Relaxation time; Sensitivity enhancement; Solid State Physics; Spectroscopy/Spectrometry; Spectrum analysis; Synthesis; Tomography; Tumors
• ISSN: 0937-9347; 1613-7507
• DOI: 10.1007/s00723-015-0756-2

Modern imaging technologies such as mMRI employ contrast agents to visualize the tumor microenvironment; therefore, demand for contrast agents, with an enhanced sensitivity and tissue tumor-specific target, is very high. The purpose of this study synthesizes novel metabolic contrast agent (Gd-DTPA-DG) and evaluates accumulation in tumor tissue for early diagnostic cancer. The contrast agent was synthesized and characterized with using different techniques including dynamic light scattering, high resolution transmission electron microscopy, Fourier transform infrared spectroscopy and inductively coupled plasma atomic emission spectroscopy (ICP-AES). Finally, MRI imaging performed to determine in vitro and in vivo relaxometry. Gd-DTPA-DG was specifically investigated in tissue tumor over a 45 min in vivo because of its ability to target metabolically active tumor tissue, and comparison with conventional contrast agent [Gd-DTPA (Magnevist, Bayer-USA)]. According to the result, the maximum image signal intensity in different concentrations (0.02 to approximately 0.8 mM), longitudinal relaxation time ( T 1 ) and transverse relaxation time ( T 2 ) were obtained. Signal intensity of tumor tissue was shown at 15 and 30 min after injection reaches maximum for Gd-DTPA-DG and Gd-DTPA (Magnevist, Bayer-USA), respectively. But Gd-DTPA-DG shown signal intensity higher of Gd-DTPA (Magnevist, Bayer-USA) over 45 min, comparison with Gd-DTPA (Magnevist, Bayer-USA). Images showed metabolic contrast agent penetrate into cells and accumulated in tumor. These results showed that the novel metabolic contrast agent could become a useful tool in early detection of cancer.