Development of novel microsatellite markers for Holothurian scabra (Holothuriidae), Apostichopus japonicas (Stichopodidae) and cross-species testing in other sea cucumbers

Thirty-five new microsatellite loci from the sea cucumbers Holothurian scabra (Jaeger, 1833) and Apostichopus japonicas (Selenka, 1867) were screened and characterized using the method of magnetic bead enrichment. Of the twenty-four polymorphic loci tested, eighteen were consistent with Hardy-Weinbe...

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Bibliographic Details
Published in:Journal of oceanology and limnology 2018-03, Vol.36 (2), p.519-527
Main Authors: Shangguan, Jingbo, Li, Zhongbao
Format: Article
Language:English
Subjects:
Alleles
Apostichopus
Aquaculture
Biology
Breeding
Earth and Environmental Science
Earth Sciences
Genetic diversity
Genetic markers
Genetic testing
Genetic variation
Genomes
Holothuroidea
Limnology
Marine invertebrates
Microsatellites
Oceanography
Polymorphism
Species
Statistical analysis
Vegetables
Wildlife conservation
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Summary:Thirty-five new microsatellite loci from the sea cucumbers Holothurian scabra (Jaeger, 1833) and Apostichopus japonicas (Selenka, 1867) were screened and characterized using the method of magnetic bead enrichment. Of the twenty-four polymorphic loci tested, eighteen were consistent with Hardy-Weinberg equilibrium after a modified false discovery rate (B-Y FDR) correction, whereas six showed statistically significant deviations (CHS2 and CHS11: P
ISSN:2096-5508
2523-3521
DOI:10.1007/s00343-018-6315-6