Transportable Multispectral Optical-Resolution Photoacoustic Microscopy Using Stimulated Raman Scattering Spectrum

• Published in: IEEE transactions on instrumentation and measurement 2024, Vol.73, p.1-9
• Main Authors: Lee, Haeni, Park, Sang Min, Park, Jeongwoo, Cho, Soon-Woo, Han, Seongyi, Ahn, Joongho, Cho, Seonghee, Kim, Chulhong, Kim, Chang-Seok, Kim, Jeesu
• Format: Article
• Language: English
• Subjects: Biomedical materials; Data acquisition; Image acquisition; Image quality; Image resolution; Imaging techniques; Medical imaging; Microscopy; Oxygen content; Photoacoustic microscopy; Raman spectra
• ISSN: 0018-9456; 1557-9662
• DOI: 10.1109/TIM.2024.3351259

Photoacoustic imaging (PAI) has become a powerful biomedical imaging technique in the last decades, combining optical and ultrasound imaging (USI) principles. Among the various configurations, optical-resolution photoacoustic microscopy (OR-PAM) served as a vital tool for high-resolution and high-sensitivity imaging of small animals in vivo. However, the current systems have limited mobility, which hinders preclinical and clinical research in cramped spaces. Here, we propose a compact ([Formula Omitted] mm3) transportable PAI system with stimulated Raman scattering (SRS) spectroscopy to address this challenge. The proposed system is designed for complete mobility, overcoming space restrictions. We demonstrate the feasibility of the system by imaging in vitro phantoms and in vivo mice. Additionally, we verify its functional imaging capabilities by visualizing oxygen saturation levels in vivo mice ears. Our results indicate that the proposed transportable PAI system maintains fast data acquisition time, superior image quality, and multispectral imaging capabilities. This shows great potential for expanding the applications of OR-PAM to a wider range of research and clinical scenarios.