Certification of a pure reference material for the ginsenoside Rg1

• Published in: Accreditation and quality assurance 2010-02, Vol.15 (2), p.81-87
• Main Authors: Kim, Dal-Ho, Chang, Jin-Kyu, Sohn, Hyun-Joo, Cho, Byong-Gu, Ko, Sung-Ryong, Nho, Kil-Bong, Jang, Dae-Sig, Lee, Sang-Myung
• Format: Article
• Language: English
• Subjects: Aerosols; Analytical Chemistry; Biochemistry; Certification; Chemical and thermal methods; Chemistry; Chemistry and Materials Science; Chromatographic methods and physical methods associated with chromatography; Commercial Law; Ecotoxicology; Electrochemical methods; Exact sciences and technology; Food Science; Gas chromatographic methods; General Paper; Herbal medicine; Homogeneity; Marketing; Mathematical models; Methods; Other chromatographic methods; Reference materials; Sensors; Shelf life; Solvents; Sulfuric acid
• ISSN: 0949-1775; 1432-0517
• DOI: 10.1007/s00769-009-0588-6

A pure certified reference material (CRM) for the ginsenoside R g 1 was prepared from roots of Panax ginseng by extraction and separation of ginsenosides. The mass fraction of the main component (ginsenoside R g 1 ) in the reference material was determined and its uncertainty was assessed from various input quantities, such as organic impurities, residual moisture, residual solvent, ash, and insoluble matters. To measure these input quantities, HPLC/CAD, Karl Fischer (KF) coulometry, gravimetry, and GC/FID were used. Homogeneity and long-term stability of the reference material are discussed. The certified mass fraction of R g 1 in the reference material is 0.974 ± 0.006 ( k  = 2) with a shelf life of 1 year.