Evaluation of measurement uncertainty in the determination of jasmonic acid in Lemna minor L. by liquid chromatography with fluorescence detection

The uncertainty was estimated for the determination of jasmonic acid (JA) content in Lemna minor L. plant extracts using a reverse-phase liquid chromatography with fluorescence detection. JA was extracted from plant material, followed by solid-phase extraction procedures, derivatisation and quantifi...

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Bibliographic Details
Published in:Accreditation and quality assurance 2007-06, Vol.12 (6), p.303-310
Main Authors: KRISTL, Janja, KRAJNCIC, Bozidar, BRODNJAK-VONCINA, Darinka, VEBER, Marjan
Format: Article
Language:English
Subjects:
Acids
Analytical chemistry
Calibration
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography
Duckweed
Exact sciences and technology
Extraction procedures
Fluorescence
Homogeneity
Jasmonic acid
Laboratories
Liquid chromatography
Other chromatographic methods
Physiology
Solid phases
Temperature effects
Uncertainty
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Summary:The uncertainty was estimated for the determination of jasmonic acid (JA) content in Lemna minor L. plant extracts using a reverse-phase liquid chromatography with fluorescence detection. JA was extracted from plant material, followed by solid-phase extraction procedures, derivatisation and quantification. In the estimation of uncertainty, the sampling, sample processing and chromatographic determination that may significantly influence the uncertainty of analytical data were considered. The results show that the method recovery and sample homogeneity are the two main contributors to uncertainty. The method has a relative expanded uncertainty (coverage factor k = 2) of about 17% at the JA content of approximately 100 ng/g.
ISSN:0949-1775
1432-0517
DOI:10.1007/s00769-007-0271-8