Electrospinning of poly (3-hydroxybutyric acid) and gelatin blended thin films: fabrication, characterization, and application in skin regeneration

A tissue engineering scaffold should mimic the structure and biological function of native extracellular matrix proteins. Electrospinning is a simple and versatile method to produce ultrathin fibers for tissue engineering. Blended submicron fibers of poly (3-hydroxybutyric acid) and gelatin were ele...

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Bibliographic Details
Published in:Polymer bulletin (Berlin, Germany) Germany), 2013-08, Vol.70 (8), p.2337-2358
Main Authors: Nagiah, Naveen, Madhavi, Lakshmi, Anitha, R., Srinivasan, Natarajan Tirupattur, Sivagnanam, Uma Tirichurapalli
Format: Article
Language:English
Subjects:
Acids
Aluminum
Applied sciences
Biocompatibility
Biological and medical sciences
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Complex Fluids and Microfluidics
Crosslinking
Electrospinning
Exact sciences and technology
Extracellular matrix
Fibers
Fibers and threads
Fibroblasts
Forms of application and semi-finished materials
Fourier transforms
Gelatin
Infrared analysis
Infrared spectroscopy
Medical sciences
Membranes
Organic Chemistry
Original Paper
Physical Chemistry
Polymer industry, paints, wood
Polymer Sciences
Polymers
Regeneration
Scaffolds
Scanning electron microscopy
Soft and Granular Matter
Spectrum analysis
Stability analysis
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Technology of polymers
Technology. Biomaterials. Equipments
Tensile strength
Thermogravimetric analysis
Thin films
Tissue engineering
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Summary:A tissue engineering scaffold should mimic the structure and biological function of native extracellular matrix proteins. Electrospinning is a simple and versatile method to produce ultrathin fibers for tissue engineering. Blended submicron fibers of poly (3-hydroxybutyric acid) and gelatin were electrospun using 1,1,1,3,3,3 hexafluoro-2-propanol as solvent. Cross linking of fibers was achieved using glutaraldehyde, and the resultant fibers were tested and analyzed using scanning electron microscopy (SEM), differential scanning calorimetry, thermogravimetric analysis, X-ray diffraction, and Fourier transformed infrared spectroscopy (FTIR).The fibers were found to exhibit good tensile strength. Degradation studies were performed and analyzed using SEM and FTIR and proved the stability of fibers for tissue engineering applications. The fibrous scaffold supported the growth and rapid proliferation of human dermal fibroblasts and keratinocytes with normal morphology, thus proving its reliability in using it as a potential scaffold for skin regeneration.
ISSN:0170-0839
1436-2449
DOI:10.1007/s00289-013-0956-6