Purification, characterization, and antioxidant activity of polysaccharides from Grifola frondosa by hydrogen peroxide/ascorbic acid-assisted extraction

To increase the production of Grifola frondosa polysaccharides (GFPs), a hydrogen peroxide/ascorbic acid (H 2 O 2 /Vc)-assisted extraction method was established. The extraction conditions were optimized by response surface methodology. Purification of the crude GFP by DEAE-52 and Sephadex G100 colu...

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Bibliographic Details
Published in:Journal of food measurement & characterization 2024, Vol.18 (1), p.797-811
Main Authors: Ding, Xi-Min, Xu, Ying-Ying, Liu, Weiming, Wang, Xingli, Tang, Meng-Ting, Zhang, Xu, Gu, Qing, Zhou, Tao
Format: Article
Language:English
Subjects:
Antioxidants
Ascorbic acid
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Chromatography
Column chromatography
Engineering
Food Science
Free radicals
Fucose
Galactose
Gene expression
Grifola frondosa
Hydrogen peroxide
Intracellular
Mannose
Methods
Molecular weight
Monosaccharides
Original Paper
Oxidative stress
Polysaccharides
Purification
Response surface methodology
Rheology
Ribose
Saccharides
Scavenging
Signal transduction
Superoxide dismutase
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Summary:To increase the production of Grifola frondosa polysaccharides (GFPs), a hydrogen peroxide/ascorbic acid (H 2 O 2 /Vc)-assisted extraction method was established. The extraction conditions were optimized by response surface methodology. Purification of the crude GFP by DEAE-52 and Sephadex G100 column chromatography yielded three fractions, GFP0, GFP1, and GFP3. Monosaccharide composition analysis revealed that these GFPs mainly consisted of mannose, glucose, galactose and l -fucose, together with a low proportion of xylose, glucuronic acid and ribose. Chemical antioxidant assays indicated that these GFPs possessed considerable reducing power and radical (DPPH · , HO · and ABTS · + ) scavenging ability. They were also observed to availably protect RAW264.7 cells from H 2 O 2 -induced oxidative stress, reduce intracellular ROS and MDA levels, and increase intracellular SOD and CAT activities. These GFPs were able to up-regulate the mRNA expression of SOD1, CAT, HO-1 and Nrf2, indicating that regulation of antioxidant enzymes and Nrf2 signaling pathway could be their important antioxidant mechanism.
ISSN:2193-4126
2193-4134
DOI:10.1007/s11694-023-02194-y