Prognostic effect of programmed cell death ligand 1/programmed cell death 1 expression in cancer stem cells of human oral squamous cell carcinoma

The relationship between cancer stem cells (CSCs) in oral squamous cell carcinoma (OSCC) and programmed cell death ligand 1 (PD-Ll)/programmed cell death 1 (PD-1) remains unclear. Therefore, the present study aimed to clarify the association between the [CD44v3.sup.high]/[CD24.sup.low] immunophenoty...

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Published in:Oncology letters 2024-02, Vol.27 (2), p.1, Article 79
Main Authors: Todoroki, Keita, Abe, Yushi, Matsuo, Katsuhisa, Nomura, Hidetosi, Kawahara, Akihiko, Nakamura, Yoshiaki, Nakamura, Moriyoshi, Seki, Naoko, Kusukawa, Jingo
Format: Article
Language:English
Subjects:
Apoptosis
Biopsy
Cancer therapies
Cell death
Chemotherapy
Cloning
Immunohistochemistry
Labeling
Ligands
Medical prognosis
Medical research
Medicine, Experimental
Metastasis
Monoclonal antibodies
Oral cancer
Patients
Phosphorylation
Radiation therapy
RNA
Squamous cell carcinoma
Stem cells
Tumors
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Summary:The relationship between cancer stem cells (CSCs) in oral squamous cell carcinoma (OSCC) and programmed cell death ligand 1 (PD-Ll)/programmed cell death 1 (PD-1) remains unclear. Therefore, the present study aimed to clarify the association between the [CD44v3.sup.high]/[CD24.sup.low] immunophenotype of CSCs in OSCC and PD-L1/PD-1 co-expression, and to assess the prognostic effect of CSCs in terms of immune checkpoint molecules. Formalin-fixed, paraffin-embedded tissue samples and clinicopathological data from 168 patients with OSCC were retrospectively retrieved. Immunohistochemical staining and reverse transcription quantitative polymerase chain reaction were applied to a tissue microarray of the invasive front of each case. Semi-automated cell counting was used to assess CD44v3, CD24, PD-L1 and PD-1 expression by immunohistochemistry (IHC) using a digital image analysis program. Associations between immunological markers and clinicopathological variables were estimated. Patients with the CSC immunophenotype [CD44v3.sup.high]/[CD24.sup.low], and patients with a high PD-L1/PD-1-positive cell density in the tumor parenchyma and stroma had significantly lower survival rates. Furthermore, patients with the CSC immunophenotype ([CD44v3.sup.high]/[CD24.sup.low]) and high PD-L1/PD-1 co-expression had even lower survival rates (P
ISSN:1792-1074
1792-1082
DOI:10.3892/ol.2024.14213