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The prospects for cryopreservation of noctuid eggs in the mass production of Trichogramma spp

Successful long-term cryopreservation of lepidopteran eggs for the mass production of parasitoids of the genus Trichogramma Westwood (Hymenoptera: Trichogrammatidae) requires a more tolerant species to withstand ultra-low temperatures. We compared the viability of eggs of Anticarsia gemmatalis Hübne...

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Published in:BioControl (Dordrecht, Netherlands) Netherlands), 2021-12, Vol.66 (6), p.753-764
Main Authors: Paixão, Magda F., Takahashi, Tamara A., Sassaki, Guilherme L., Foerster, Luís A.
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description Successful long-term cryopreservation of lepidopteran eggs for the mass production of parasitoids of the genus Trichogramma Westwood (Hymenoptera: Trichogrammatidae) requires a more tolerant species to withstand ultra-low temperatures. We compared the viability of eggs of Anticarsia gemmatalis Hübner (Lepidoptera: Erebidae) and Mythimna sequax Franclemont (Lepidoptera: Noctuidae), cryopreserved in liquid nitrogen, and evaluated the viability of M. sequax eggs to support the large-scale production of high-quality Trichogramma species. The presence of anti-freezing metabolites in lepidopteran eggs was detected and quantified by 1 H nuclear magnetic resonance (NMR) spectroscopy. Parasitism by Trichogramma pretiosum Riley of M. sequax eggs stored for 30 days was significantly higher (84.2%) in comparison to A. gemmatalis (6.7%). The 1 H NMR spectrum showed that the amounts of anti-freezing metabolites maltodextrin and trehalose were greater in M. sequax eggs, a possible explanation for their suitability to cryopreservation in comparison to A. gemmatalis . Eggs of M. sequax cryopreserved for up to 12 months maintained a mean rate of parasitism between 80 and 90% by Trichogramma atopovirilia Oatman and Platner and T. pretiosum, the latter declining to 79% after 12 months. Both Trichogramma species can exploit long-term storage of M. sequax eggs at ultra-low temperatures, without any apparent fitness penalty to the first generation. This is the first record of eggs of a noctuid species being cryopreserved for up to one year and remaining susceptible to parasitism by Trichogramma species. This procedure should enable large-scale production of hosts with high nutritional quality, and the production of parasitoids that is synchronized better with their use as biological control agents in annual crops.
doi_str_mv 10.1007/s10526-021-10109-3
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ispartof BioControl (Dordrecht, Netherlands), 2021-12, Vol.66 (6), p.753-764
issn 1386-6141
1573-8248
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source Springer Nature
subjects Agriculture
Animal Biochemistry
Animal Ecology
Behavioral Sciences
Biological control
Biomedical and Life Sciences
Butterflies & moths
Cryopreservation
Eggs
Entomology
Females
Freezing
Insects
Lepidoptera
Life Sciences
Liquid nitrogen
Low temperature
Maltodextrin
Mass production
Metabolites
New records
Nitrogen
NMR
NMR spectroscopy
Nuclear magnetic resonance
Nutritive value
Parasitism
Plant Pathology
Species
Spectroscopy
Spectrum analysis
Trehalose
Trichogramma
Wheat
title The prospects for cryopreservation of noctuid eggs in the mass production of Trichogramma spp
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