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The prospects for cryopreservation of noctuid eggs in the mass production of Trichogramma spp
Successful long-term cryopreservation of lepidopteran eggs for the mass production of parasitoids of the genus Trichogramma Westwood (Hymenoptera: Trichogrammatidae) requires a more tolerant species to withstand ultra-low temperatures. We compared the viability of eggs of Anticarsia gemmatalis Hübne...
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Published in: | BioControl (Dordrecht, Netherlands) Netherlands), 2021-12, Vol.66 (6), p.753-764 |
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creator | Paixão, Magda F. Takahashi, Tamara A. Sassaki, Guilherme L. Foerster, Luís A. |
description | Successful long-term cryopreservation of lepidopteran eggs for the mass production of parasitoids of the genus
Trichogramma
Westwood (Hymenoptera: Trichogrammatidae) requires a more tolerant species to withstand ultra-low temperatures. We compared the viability of eggs of
Anticarsia gemmatalis
Hübner (Lepidoptera: Erebidae) and
Mythimna sequax
Franclemont (Lepidoptera: Noctuidae), cryopreserved in liquid nitrogen, and evaluated the viability of
M. sequax
eggs to support the large-scale production of high-quality
Trichogramma
species. The presence of anti-freezing metabolites in lepidopteran eggs was detected and quantified by
1
H nuclear magnetic resonance (NMR) spectroscopy. Parasitism by
Trichogramma pretiosum
Riley of
M. sequax
eggs stored for 30 days was significantly higher (84.2%) in comparison to
A. gemmatalis
(6.7%). The
1
H NMR spectrum showed that the amounts of anti-freezing metabolites maltodextrin and trehalose were greater in
M. sequax
eggs, a possible explanation for their suitability to cryopreservation in comparison to
A. gemmatalis
. Eggs of
M. sequax
cryopreserved for up to 12 months maintained a mean rate of parasitism between 80 and 90% by
Trichogramma atopovirilia
Oatman and Platner and
T. pretiosum,
the latter declining to 79% after 12 months. Both
Trichogramma
species can exploit long-term storage of
M. sequax
eggs at ultra-low temperatures, without any apparent fitness penalty to the first generation. This is the first record of eggs of a noctuid species being cryopreserved for up to one year and remaining susceptible to parasitism by
Trichogramma
species. This procedure should enable large-scale production of hosts with high nutritional quality, and the production of parasitoids that is synchronized better with their use as biological control agents in annual crops. |
doi_str_mv | 10.1007/s10526-021-10109-3 |
format | article |
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Trichogramma
Westwood (Hymenoptera: Trichogrammatidae) requires a more tolerant species to withstand ultra-low temperatures. We compared the viability of eggs of
Anticarsia gemmatalis
Hübner (Lepidoptera: Erebidae) and
Mythimna sequax
Franclemont (Lepidoptera: Noctuidae), cryopreserved in liquid nitrogen, and evaluated the viability of
M. sequax
eggs to support the large-scale production of high-quality
Trichogramma
species. The presence of anti-freezing metabolites in lepidopteran eggs was detected and quantified by
1
H nuclear magnetic resonance (NMR) spectroscopy. Parasitism by
Trichogramma pretiosum
Riley of
M. sequax
eggs stored for 30 days was significantly higher (84.2%) in comparison to
A. gemmatalis
(6.7%). The
1
H NMR spectrum showed that the amounts of anti-freezing metabolites maltodextrin and trehalose were greater in
M. sequax
eggs, a possible explanation for their suitability to cryopreservation in comparison to
A. gemmatalis
. Eggs of
M. sequax
cryopreserved for up to 12 months maintained a mean rate of parasitism between 80 and 90% by
Trichogramma atopovirilia
Oatman and Platner and
T. pretiosum,
the latter declining to 79% after 12 months. Both
Trichogramma
species can exploit long-term storage of
M. sequax
eggs at ultra-low temperatures, without any apparent fitness penalty to the first generation. This is the first record of eggs of a noctuid species being cryopreserved for up to one year and remaining susceptible to parasitism by
Trichogramma
species. This procedure should enable large-scale production of hosts with high nutritional quality, and the production of parasitoids that is synchronized better with their use as biological control agents in annual crops.</description><identifier>ISSN: 1386-6141</identifier><identifier>EISSN: 1573-8248</identifier><identifier>DOI: 10.1007/s10526-021-10109-3</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Agriculture ; Animal Biochemistry ; Animal Ecology ; Behavioral Sciences ; Biological control ; Biomedical and Life Sciences ; Butterflies & moths ; Cryopreservation ; Eggs ; Entomology ; Females ; Freezing ; Insects ; Lepidoptera ; Life Sciences ; Liquid nitrogen ; Low temperature ; Maltodextrin ; Mass production ; Metabolites ; New records ; Nitrogen ; NMR ; NMR spectroscopy ; Nuclear magnetic resonance ; Nutritive value ; Parasitism ; Plant Pathology ; Species ; Spectroscopy ; Spectrum analysis ; Trehalose ; Trichogramma ; Wheat</subject><ispartof>BioControl (Dordrecht, Netherlands), 2021-12, Vol.66 (6), p.753-764</ispartof><rights>International Organization for Biological Control (IOBC) 2021</rights><rights>International Organization for Biological Control (IOBC) 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c319t-29e40725e5a5356a5c86bcbc3f50dfc1d2d60727a9dddd351ed2a882863f8e313</citedby><cites>FETCH-LOGICAL-c319t-29e40725e5a5356a5c86bcbc3f50dfc1d2d60727a9dddd351ed2a882863f8e313</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Paixão, Magda F.</creatorcontrib><creatorcontrib>Takahashi, Tamara A.</creatorcontrib><creatorcontrib>Sassaki, Guilherme L.</creatorcontrib><creatorcontrib>Foerster, Luís A.</creatorcontrib><title>The prospects for cryopreservation of noctuid eggs in the mass production of Trichogramma spp</title><title>BioControl (Dordrecht, Netherlands)</title><addtitle>BioControl</addtitle><description>Successful long-term cryopreservation of lepidopteran eggs for the mass production of parasitoids of the genus
Trichogramma
Westwood (Hymenoptera: Trichogrammatidae) requires a more tolerant species to withstand ultra-low temperatures. We compared the viability of eggs of
Anticarsia gemmatalis
Hübner (Lepidoptera: Erebidae) and
Mythimna sequax
Franclemont (Lepidoptera: Noctuidae), cryopreserved in liquid nitrogen, and evaluated the viability of
M. sequax
eggs to support the large-scale production of high-quality
Trichogramma
species. The presence of anti-freezing metabolites in lepidopteran eggs was detected and quantified by
1
H nuclear magnetic resonance (NMR) spectroscopy. Parasitism by
Trichogramma pretiosum
Riley of
M. sequax
eggs stored for 30 days was significantly higher (84.2%) in comparison to
A. gemmatalis
(6.7%). The
1
H NMR spectrum showed that the amounts of anti-freezing metabolites maltodextrin and trehalose were greater in
M. sequax
eggs, a possible explanation for their suitability to cryopreservation in comparison to
A. gemmatalis
. Eggs of
M. sequax
cryopreserved for up to 12 months maintained a mean rate of parasitism between 80 and 90% by
Trichogramma atopovirilia
Oatman and Platner and
T. pretiosum,
the latter declining to 79% after 12 months. Both
Trichogramma
species can exploit long-term storage of
M. sequax
eggs at ultra-low temperatures, without any apparent fitness penalty to the first generation. This is the first record of eggs of a noctuid species being cryopreserved for up to one year and remaining susceptible to parasitism by
Trichogramma
species. This procedure should enable large-scale production of hosts with high nutritional quality, and the production of parasitoids that is synchronized better with their use as biological control agents in annual crops.</description><subject>Agriculture</subject><subject>Animal Biochemistry</subject><subject>Animal Ecology</subject><subject>Behavioral Sciences</subject><subject>Biological control</subject><subject>Biomedical and Life Sciences</subject><subject>Butterflies & moths</subject><subject>Cryopreservation</subject><subject>Eggs</subject><subject>Entomology</subject><subject>Females</subject><subject>Freezing</subject><subject>Insects</subject><subject>Lepidoptera</subject><subject>Life Sciences</subject><subject>Liquid nitrogen</subject><subject>Low temperature</subject><subject>Maltodextrin</subject><subject>Mass production</subject><subject>Metabolites</subject><subject>New records</subject><subject>Nitrogen</subject><subject>NMR</subject><subject>NMR spectroscopy</subject><subject>Nuclear magnetic resonance</subject><subject>Nutritive value</subject><subject>Parasitism</subject><subject>Plant Pathology</subject><subject>Species</subject><subject>Spectroscopy</subject><subject>Spectrum analysis</subject><subject>Trehalose</subject><subject>Trichogramma</subject><subject>Wheat</subject><issn>1386-6141</issn><issn>1573-8248</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LAzEQhoMoWKt_wFPAczSTNNnsUYpaoeClHiWk2WS7xd2sya7Qf2_qKt6cywzM-8zHi9A10FugtLhLQAWThDIgQIGWhJ-gGYiCE8UW6jTXXEkiYQHn6CKlPc2QEGqG3jY7h_sYUu_skLAPEdt4CH10ycVPMzShw8HjLthhbCrs6jrhpsNDplqT0hGtRvsr28TG7kIdTdsanPr-Ep15857c1U-eo9fHh81yRdYvT8_L-zWxHMqBsNItaMGEE0ZwIY2wSm7t1nIvaOUtVKySuV-YssrBBbiKGaWYktwrx4HP0c00N5_zMbo06H0YY5dXalaCYowWnGcVm1Q2_5ui87qPTWviQQPVRxv1ZKPONupvG_UR4hOUsrirXfwb_Q_1Bcv1dl0</recordid><startdate>20211201</startdate><enddate>20211201</enddate><creator>Paixão, Magda F.</creator><creator>Takahashi, Tamara A.</creator><creator>Sassaki, Guilherme L.</creator><creator>Foerster, Luís A.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7SN</scope><scope>7SS</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope></search><sort><creationdate>20211201</creationdate><title>The prospects for cryopreservation of noctuid eggs in the mass production of Trichogramma spp</title><author>Paixão, Magda F. ; 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Trichogramma
Westwood (Hymenoptera: Trichogrammatidae) requires a more tolerant species to withstand ultra-low temperatures. We compared the viability of eggs of
Anticarsia gemmatalis
Hübner (Lepidoptera: Erebidae) and
Mythimna sequax
Franclemont (Lepidoptera: Noctuidae), cryopreserved in liquid nitrogen, and evaluated the viability of
M. sequax
eggs to support the large-scale production of high-quality
Trichogramma
species. The presence of anti-freezing metabolites in lepidopteran eggs was detected and quantified by
1
H nuclear magnetic resonance (NMR) spectroscopy. Parasitism by
Trichogramma pretiosum
Riley of
M. sequax
eggs stored for 30 days was significantly higher (84.2%) in comparison to
A. gemmatalis
(6.7%). The
1
H NMR spectrum showed that the amounts of anti-freezing metabolites maltodextrin and trehalose were greater in
M. sequax
eggs, a possible explanation for their suitability to cryopreservation in comparison to
A. gemmatalis
. Eggs of
M. sequax
cryopreserved for up to 12 months maintained a mean rate of parasitism between 80 and 90% by
Trichogramma atopovirilia
Oatman and Platner and
T. pretiosum,
the latter declining to 79% after 12 months. Both
Trichogramma
species can exploit long-term storage of
M. sequax
eggs at ultra-low temperatures, without any apparent fitness penalty to the first generation. This is the first record of eggs of a noctuid species being cryopreserved for up to one year and remaining susceptible to parasitism by
Trichogramma
species. This procedure should enable large-scale production of hosts with high nutritional quality, and the production of parasitoids that is synchronized better with their use as biological control agents in annual crops.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s10526-021-10109-3</doi><tpages>12</tpages></addata></record> |
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source | Springer Nature |
subjects | Agriculture Animal Biochemistry Animal Ecology Behavioral Sciences Biological control Biomedical and Life Sciences Butterflies & moths Cryopreservation Eggs Entomology Females Freezing Insects Lepidoptera Life Sciences Liquid nitrogen Low temperature Maltodextrin Mass production Metabolites New records Nitrogen NMR NMR spectroscopy Nuclear magnetic resonance Nutritive value Parasitism Plant Pathology Species Spectroscopy Spectrum analysis Trehalose Trichogramma Wheat |
title | The prospects for cryopreservation of noctuid eggs in the mass production of Trichogramma spp |
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