Comparative Anticancer Potential of Biologically and Chemically Synthesized Gold Nanoparticles

The present study was aimed to evaluate the anticancer behaviour of the biogenic and chemically synthesized gold nanoparticles (GNPs) towards cancerous (HeLa, MCF-7, A549 and H1299) and normal (HEK293) cell lines. We observed that biologically synthesized particles were spherical with average size r...

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Bibliographic Details
Published in:Journal of cluster science 2020-07, Vol.31 (4), p.867-876
Main Authors: Virmani, Ishita, Sasi, Christo, Priyadarshini, Eepsita, Kumar, Raj, Sharma, Saurabh Kumar, Singh, Gajendra Pratap, Pachwarya, Ram Babu, Paulraj, R., Barabadi, Hamed, Saravanan, Muthupandian, Meena, Ramovatar
Format: Article
Language:English
Subjects:
Cancer therapies
Catalysis
Cervical cancer
Chemicals
Chemistry
Chemistry and Materials Science
Chemotherapy
Deregulation
Electron microscopes
Gold
Inorganic Chemistry
Malaria
Metabolites
Morphology
Nanochemistry
Nanoparticles
Original Paper
Physical Chemistry
Proteins
Substrates
Synthesis
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Summary:The present study was aimed to evaluate the anticancer behaviour of the biogenic and chemically synthesized gold nanoparticles (GNPs) towards cancerous (HeLa, MCF-7, A549 and H1299) and normal (HEK293) cell lines. We observed that biologically synthesized particles were spherical with average size ranging from 2 to 10 nm, whereas the chemically synthesized particles were polydispersed with average size ranging from 5 to 20 nm. SEM analysis suggested the crystalline behaviour of the synthesized particles. Imaging results revealed that the particles were able to penetrate the cells with localization in cytosol or in the nucleus. Cell viability assay suggested the biologically synthesized particles significantly inhibited the growth of cancerous cells in a dose dependent manner with an IC 50 value of around 200 µg/mL and were found to be non-toxic towards normal cells (HEK293). While the citrate capped particles were observed to be able to kill only around 20–25% of cells even at a high concentration of 400 µg/mL. Colony suppression studies, intracellular ROS estimation, cell cycle arrest and deregulation of mitochondrial membrane potential showed the high anticancer efficiency of biologically synthesized particles in comparison to chemically synthesized particles. Based on these observations we confirm Ocimum tenuiflorum extract stabilized GNPs have potentially promising antitumor substrates.
ISSN:1040-7278
1572-8862
DOI:10.1007/s10876-019-01695-5