Biogenic Gold Nanoparticles from Fusarium oxysporum: The Impact of Fungal Morphology and Localization Studies

The synthesis of nanoparticles from biological sources has gained significant attention recently, however, with much emphasis on the novelty of sources. This study aims to provide a comprehensive overview on the aspects of fungal age and type of cultivation on the properties of the nanoparticles. Th...

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Bibliographic Details
Published in:Journal of cluster science 2020-11, Vol.31 (6), p.1185-1197
Main Authors: Deepa, Kannan, Panda, Tapobrata
Format: Article
Language:English
Subjects:
Age factors
Biocompatibility
Biosynthesis
Catalysis
Chemistry
Chemistry and Materials Science
Cytoplasm
Electron microscopes
Fungi
Fusarium oxysporum
Gold
Icosahedrons
Image enhancement
Inorganic Chemistry
Localization
Metabolites
Microorganisms
Microscopy
Morphology
Nanochemistry
Nanomaterials
Nanoparticles
Original Paper
Physical Chemistry
Physiology
Saline solutions
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Summary:The synthesis of nanoparticles from biological sources has gained significant attention recently, however, with much emphasis on the novelty of sources. This study aims to provide a comprehensive overview on the aspects of fungal age and type of cultivation on the properties of the nanoparticles. The effect of age of Fusarium oxysporum developed in submerged culture and on solid agar medium was evaluated. When the gold salt solution was added to the fungal colony on agar medium, a deep purple colored radial zone developed in the 3 days old mycelium. The nanoparticles in the radial purple zone had two distinct characteristics when the agar blocks were dried by either lyophilization or heating in a hot air oven. The lyophilized sample contained square bi-pyramidal particles (1 to 2 μm). The oven-dried sample contained abundant truncated triangular nanoplates (150 to 500 nm), decahedrons and icosahedrons (20 to 70 nm), and a few triangular and hexagonal nanoplates (50 nm). The mycelium when suspended in 1 mM HAuCl 4 ·3H 2 O presented distinct geometries of the nanoparticles in the cytoplasm and periplasm. The formation of gold nanoparticles of different morphologies within the cytoplasm, and the periplasm has implications in Surface Enhanced Raman Spectroscopic (SERS) measurements. Graphic Abstract HRTEM images of hyphae of Fusarium oxysporum suspended in 1 mM HAuCl 4 ·3H 2 O for 24 h. The nanoparticles present in the electron dense cytoplasm are markedly different from those in the periplasmic space.
ISSN:1040-7278
1572-8862
DOI:10.1007/s10876-019-01725-2