Nanoscale Chlorophyll-Liposome Composite (NCLC) Fluorescent Probe for In Vivo Bio-imaging

Green technology for in vivo bioimaging is being developed for its biocompatibility and efficacy to serve as fluorescent probes in interior environment of the system. Herein, the preparation of nanoscale chlorophyll-liposome composite (NCLC) from the leaf extract of Tridax procumbens having near IR...

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Bibliographic Details
Published in:Journal of cluster science 2017-09, Vol.28 (5), p.2969-2977
Main Authors: Uma Suganya, K. S., Govindaraju, K., Veena Vani, C., Kirubagaran, R., Ashok Kumar, T., Tamilselvan, S., Veeramani, V., Ganesh Kumar, V.
Format: Article
Language:English
Subjects:
Antibodies
Aquaculture
Biocompatibility
Catalysis
Chemistry
Chemistry and Materials Science
Chlorophyll
Conjugation
Cysts
Emission analysis
Ethanol
Field emission microscopy
Field emission spectroscopy
Fluorescent indicators
Fourier transforms
Infrared spectroscopy
Inorganic Chemistry
Liposomes
Medical imaging
Molecular weight
Nanochemistry
Nanocomposites
Nanomaterials
Original Paper
Physical Chemistry
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Summary:Green technology for in vivo bioimaging is being developed for its biocompatibility and efficacy to serve as fluorescent probes in interior environment of the system. Herein, the preparation of nanoscale chlorophyll-liposome composite (NCLC) from the leaf extract of Tridax procumbens having near IR fluorescence is reported. The prepared NCLC has been characterized and confirmed using UV–vis spectroscopy, Fluorescence spectroscopy, Fourier transform infrared spectroscopy and Field emission-Scanning electron microscopic studies. Fluorescence microscopic images of Artemia nauplii and Penaeus vannamei after treatment with NCLC revealed its uptake evident from the appearance of red fluorescent granules in its alimentary canal which infers its possible utilization as an in vivo bioimaging tool (nanoprobes) on conjugation with specific antibodies for disease diagnosis in aquaculture.
ISSN:1040-7278
1572-8862
DOI:10.1007/s10876-017-1272-3