Development and Characterization of Phytoniosome Nano Vesicle Loaded with Aqueous Leaf Extracts of Justicia adhatoda and Psidium guajoava Against Dengue Virus (DEN-2)

• Published in: Journal of cluster science 2021-03, Vol.32 (2), p.297-304
• Main Authors: Wilson, Dhanya K., Shyamala, Govindarajan, Paulpandi, Manickam, Narayanasamy, Arul, Siram, Karthik, Karuppaiah, Arjunan, Sankar, Veintramuthu
• Format: Article
• Language: English
• Subjects: Catalysis; Chemistry; Chemistry and Materials Science; Cholesterol; Dengue fever; Drug delivery systems; Flavonoids; Herbal medicine; Hydration; Infections; Infectious diseases; Inorganic Chemistry; Leaves; Nanochemistry; Original Paper; Particle size; Physical Chemistry; Poloxamers; Polyethylene glycol; Reagents; Room temperature; Surfactants; Thin films; Vaccines; Viruses
• ISSN: 1040-7278; 1572-8862
• DOI: 10.1007/s10876-020-01788-6

The leaves of Psidium guajava (PG) and Justicia adhatoda (JA) are traditionally used in the Indian herbal medicine for the treatment of dengue. The current research work was aimed to evaluate the antiviral activity of the phytoniosomes (PN) loaded with the aqueous extracts of PG and JA (AGN) against dengue virus-2 strain (DEN-2). The AGN were prepared by ultrasonication method using tween 80, poloxamer 407, polyethylene glycol 6000 and cholesterol. The particle size of the PN was found out to be in the range of 105.1 ± 3.6–279.4 ± 5.2 nm. In comparison to the non-PEGylated niosomes, PEGylated niosomes showed lower particle size. AGN showed in vitro antiviral activity against vero cells infected with DEN-2 virus. Capsules containing AGN powder were found to be stable for 3 months when stored at room temperature. AGN4 showed maximum of 40% plaque forming units, indicating that formulation poses significant viral inhibition in in vitro condition.