5-Ethynyl-2＇-deoxyuridine as a molecular probe of cell proliferation for high-content siRNA screening assay by ＂click＂ chemistry

Labelling and identification of proliferating cells is important for the study of physiological or pathological processes in high-content screening （HCS） assays. Here we describe ethynyl deoxyuridine （EdU） as a biomarker for the assessment of cell proliferation and clearly demonstrate the feasibilit...

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Bibliographic Details
Published in:Science China. Chemistry 2011-11, Vol.54 (11), p.1702-1710
Main Authors: Chen, MiaoJuan, Qu, DeZhong, Chi, WeiLin, Wang, Wei, Ren, XiaoShuai, Cong, ShuJie, Liang, PeiZhou, Feng, ShiPeng, Zhang, BiLiang
Format: Article
Language:English
Subjects:
Assaying
Biomarkers
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Labeling
Screening
siRNA
乙炔
分子探针
化学
尿苷
筛选检测
细胞增殖
脱氧
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Summary:Labelling and identification of proliferating cells is important for the study of physiological or pathological processes in high-content screening （HCS） assays. Here we describe ethynyl deoxyuridine （EdU） as a biomarker for the assessment of cell proliferation and clearly demonstrate the feasibility of the EdU-labelling method for use in HCS assays. EdU detection is highly robust, reproducible, technically simple, and well suited for automated segmentation, which provides an excellent al- ternative for setting up multiplexed HCS assays of siRNA, miRNA and small-molecule libraries.
ISSN:1674-7291
1869-1870
DOI:10.1007/s11426-011-4398-4