Label-free detection repeatability of protein microarrays by oblique-incidence reflectivity difference method

We examine the repeatabilities of oblique-incidence reflectivity difference（OIRD） method for label-free detecting biological molecular interaction using protein microarrays.The experimental results show that the repeatabilities are the same in a given microarray or microarray-microarray and are cons...

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Bibliographic Details
Published in:Science China. Physics, mechanics & astronomy mechanics & astronomy, 2012-12, Vol.55 (12), p.2347-2350
Main Authors: Dai, Jun, Li, Lin, Wang, JingYi, He, LiPing, Lu, HuiBin, Ruan, KangCheng, Jin, KuiJuan, Yang, GuoZhen
Format: Article
Language:English
Subjects:
Astronomy
Classical and Continuum Physics
Labels
Molecular interactions
Observations and Techniques
Physics
Physics and Astronomy
Proteins
Reflectance
反射率
可重复性
斜入射
无标记
检测
生物芯片技术
蛋白质微阵列
蛋白质芯片
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Summary:We examine the repeatabilities of oblique-incidence reflectivity difference（OIRD） method for label-free detecting biological molecular interaction using protein microarrays.The experimental results show that the repeatabilities are the same in a given microarray or microarray-microarray and are consistent,indicating that OIRD is a promising label-free detection technique for biological microarrays.
ISSN:1674-7348
1869-1927
DOI:10.1007/s11433-012-4952-x