Rapid and sensitive determination of fatty acids in edible oil by liquid chromatography-electrospray ionization tandem mass spectrometry

• Published in: Science China. Chemistry 2014-03, Vol.57 (3), p.447-452
• Main Authors: Bi, Xin, Jin, YiBao, Li, ShangFu, Gao, Dan, Jiang, YuYang, Liu, HongXia
• Format: Article
• Language: English
• Subjects: Acetonitrile; Ammonium acetate; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Chloroform; Docosahexaenoic acid; Edible oils; Electrospraying; Fatty acids; High performance liquid chromatography; Ionization; Isopropanol; Mass spectrometry; 串联质谱法; 定量测定; 灵敏; 电喷雾电离质谱; 脂肪酸; 色谱; 质谱法; 食用油; 高效液相色谱法
• ISSN: 1674-7291; 1869-1870
• DOI: 10.1007/s11426-013-4934-5

A sensitive and robust on-line LC/MS method was developed for quantitative determination of linoleic acid,docosahexaenoic acid and docosanoic acid from edible oil samples.The oil samples were dissolved in chloroform-isopropyl alcohol（20：80,v：v）solution and the three fatty acids were separated by HPLC with a C4 column using 10 mmol/L ammonium acetate-isopropyl alcohol-acetonitrile（20：40：40,v：v：v）mobile phase in isocratic elution.Electrospray ionization mass spectrometry with the selected ion recording monitoring was used to detect and quantify the fatty acid.The calibration curves were linear in the range of 10.00–5000 pg/mL for linoleic acid and docosanoic acid,and 1.000–500.0 pg/mL for docosahexaenoic acid.The limit of detection was 2.0 pg/mL for linoleic acid,3.0 pg/mL for docosanoic acid,and 0.20 pg/mL for docosahexaenoic acid.The results showed that the method described in this paper could be utilized for rapid determination of three fatty acids at picogram levels in edible oils.