Recent advances in the preparation of Fmoc-SPPS-based peptide thioester and its surrogates for NCL-type reactions

Solid phase peptide synthesis（SPPS）based on Fmoc chemistry has become a commonly used technique in peptide chemistry,as it can be easily conducted using automated machine,and not requiring highly toxic HF in comparison to Boc-SPPS.With the fast development in the emerging field of protein chemical s...

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Bibliographic Details
Published in:Science China. Chemistry 2017-02, Vol.60 (2), p.201-213
Main Authors: Li, Hongxing, Dong, Suwei
Format: Article
Language:English
Subjects:
Amino acids
Automation
Chemical reactions
Chemical synthesis
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Condensation
Fmoc
Fragments
NCL
Peptides
Proteins
Reviews
Solid phases
Solvents
代理人
制备
化学合成
反应
固相多肽合成
硫酯
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Summary:Solid phase peptide synthesis（SPPS）based on Fmoc chemistry has become a commonly used technique in peptide chemistry,as it can be easily conducted using automated machine,and not requiring highly toxic HF in comparison to Boc-SPPS.With the fast development in the emerging field of protein chemical synthesis,many efforts have been endeavored aiming to find more efficient methods for preparing peptide fragments required in ligation reactions.This review briefly summarizes recent advances in the engineering and modification of Fmoc-SPPS-derived peptides,which can be used as the N-terminal fragments in a native chemical ligation（NCL）or NCL-type ligation reactions.
ISSN:1674-7291
1869-1870
DOI:10.1007/s11426-016-0381-1