Enhanced dSTORM imaging using fluorophores interacting with cucurbituril

Advanced fluorescence microscopy including single-molecule localization-based super-resolution imaging techniques requires bright and photostable dyes or proteins as fluorophores. The photophysical properties of fluorophores have been proven to be crucial for super-resolution microscopy＇s localizati...

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Bibliographic Details
Published in:Science China. Chemistry 2016-07, Vol.59 (7), p.848-852
Main Authors: Zhang, Min, Gao, Jing, Chen, Junling, Cai, Mingjun, Jiang, Junguang, Tian, Zhiyuan, Wang, Hongda
Format: Article
Language:English
Subjects:
Chemical compounds
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Communications
Dyes
Fluorescence
Image resolution
Imaging techniques
Localization
Microscopy
Supramolecular compounds
光物理性质
定位精度
成像技术
荧光增强
荧光显微镜
荧光蛋白
葫芦脲
超分辨率
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Summary:Advanced fluorescence microscopy including single-molecule localization-based super-resolution imaging techniques requires bright and photostable dyes or proteins as fluorophores. The photophysical properties of fluorophores have been proven to be crucial for super-resolution microscopy＇s localization precision and imaging resolution. Fluorophores TAMRA and Atto Rho6G, which can interact with macrocyclic host cucurbit[7]uril （CB7） to form host-guest compounds, were found to improve the fluorescence intensity and lifetimes of these dyes. We enhanced the localization precision of direct stochastic optical reconstruction microscopy （dSTORM） by introducing CB7 into the imaging buffer, and showed that the number of photons as well as localizations of both TAMRA and Atto Rho6G increase over 2 times.
ISSN:1674-7291
1869-1870
DOI:10.1007/s11426-015-0535-0