Computational analysis of triplex formation of oligonucleotides:protonated and 5-methylated py-pu-py motif

A triple helix composed of three dodecanucleotides,5’-d(TC)6,5-(AG)6 and 3’-d(TC)6 with each containing a methylcytosme or a protonated-cylosine,was examined in terms of various interactions Computational malvsis of the stability of triplex formation was consistent with the experimental observations...

Full description

Saved in:
Bibliographic Details
Published in:Science China. Chemistry 1997-04, Vol.40 (2), p.113-121
Main Author: 白春礼 王琛 汪新文
Format: Article
Language:English
Subjects:
analysis
computational
DNA
effect
Oligonucleotides
Protonation
Screening
stability
Stability analysis
triplex
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c285t-df47cc91a238ce9a0494fc218e8c1c0c9b5744a0de9bdab777a265341d0645be3
cites cdi_FETCH-LOGICAL-c285t-df47cc91a238ce9a0494fc218e8c1c0c9b5744a0de9bdab777a265341d0645be3
container_end_page 121
container_issue 2
container_start_page 113
container_title Science China. Chemistry
container_volume 40
creator 白春礼 王琛 汪新文
description A triple helix composed of three dodecanucleotides,5’-d(TC)6,5-(AG)6 and 3’-d(TC)6 with each containing a methylcytosme or a protonated-cylosine,was examined in terms of various interactions Computational malvsis of the stability of triplex formation was consistent with the experimental observations.The Hoogsleen hase among interaction was increased through substitution of 5-methylcytosme for cytosme and/or protonation The inter-woon between water molecules and DNA triplex was visualized in terms,of the end-to-end distance change of each trand The chain length was reduced by about 7 5%-16 2% due to the screening effect In addition,as the screening fleer nerenises,Hoogsteen base pairs appear to be stable in triplex formation.Our computational results also show this the effect of protonated- and methylated cytosme on the stability of triplex formation was due to the change of the beerostanis mteractions rather than the surface stacking interaction
doi_str_mv 10.1007/BF02876401
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2918663992</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cqvip_id>3001013942</cqvip_id><sourcerecordid>2918663992</sourcerecordid><originalsourceid>FETCH-LOGICAL-c285t-df47cc91a238ce9a0494fc218e8c1c0c9b5744a0de9bdab777a265341d0645be3</originalsourceid><addsrcrecordid>eNpFkE1PwzAMhiMEEtPYhV9QiRtSIF9tEm4wMUCaxAXOVZomW6e26ZJUov-ebEPMB9uyH1v2C8AtRg8YIf74skJE8IIhfAFmWBQSYsHRZcoLziAnEl-DRQg7lIxSRHg-A7ul64Yxqti4XrWZSm4KTciczaJvhtb8ZNb57tg_FF3bbFw_6ta42NQmPA3exTQaTZ2G6yyHnYnbqT0WhgkOIxymrEuwvQFXVrXBLP7iHHyvXr-W73D9-faxfF5DTUQeYW0Z11piRajQRirEJLOaYGGExhppWeWcMYVqI6taVZxzRYqcMlyjguWVoXNwd9qbTtuPJsRy50afHgtlkkAUBZWSJOr-RGnvQvDGloNvOuWnEqPyIGd5lvO8Um9dv9k3_eafpgjhREhG6C8HGnRl</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2918663992</pqid></control><display><type>article</type><title>Computational analysis of triplex formation of oligonucleotides:protonated and 5-methylated py-pu-py motif</title><source>Springer Nature</source><creator>白春礼 王琛 汪新文</creator><creatorcontrib>白春礼 王琛 汪新文</creatorcontrib><description>A triple helix composed of three dodecanucleotides,5’-d(TC)6,5-(AG)6 and 3’-d(TC)6 with each containing a methylcytosme or a protonated-cylosine,was examined in terms of various interactions Computational malvsis of the stability of triplex formation was consistent with the experimental observations.The Hoogsleen hase among interaction was increased through substitution of 5-methylcytosme for cytosme and/or protonation The inter-woon between water molecules and DNA triplex was visualized in terms,of the end-to-end distance change of each trand The chain length was reduced by about 7 5%-16 2% due to the screening effect In addition,as the screening fleer nerenises,Hoogsteen base pairs appear to be stable in triplex formation.Our computational results also show this the effect of protonated- and methylated cytosme on the stability of triplex formation was due to the change of the beerostanis mteractions rather than the surface stacking interaction</description><identifier>ISSN: 1674-7291</identifier><identifier>ISSN: 1006-9291</identifier><identifier>EISSN: 1869-1870</identifier><identifier>EISSN: 1862-2771</identifier><identifier>DOI: 10.1007/BF02876401</identifier><language>eng</language><publisher>Heidelberg: Springer Nature B.V</publisher><subject>analysis ; computational ; DNA ; effect ; Oligonucleotides ; Protonation ; Screening ; stability ; Stability analysis ; triplex</subject><ispartof>Science China. Chemistry, 1997-04, Vol.40 (2), p.113-121</ispartof><rights>Science in China Press 1997.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c285t-df47cc91a238ce9a0494fc218e8c1c0c9b5744a0de9bdab777a265341d0645be3</citedby><cites>FETCH-LOGICAL-c285t-df47cc91a238ce9a0494fc218e8c1c0c9b5744a0de9bdab777a265341d0645be3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/60113X/60113X.jpg</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids></links><search><creatorcontrib>白春礼 王琛 汪新文</creatorcontrib><title>Computational analysis of triplex formation of oligonucleotides:protonated and 5-methylated py-pu-py motif</title><title>Science China. Chemistry</title><addtitle>SCIENCE CHINA Chemistry</addtitle><description>A triple helix composed of three dodecanucleotides,5’-d(TC)6,5-(AG)6 and 3’-d(TC)6 with each containing a methylcytosme or a protonated-cylosine,was examined in terms of various interactions Computational malvsis of the stability of triplex formation was consistent with the experimental observations.The Hoogsleen hase among interaction was increased through substitution of 5-methylcytosme for cytosme and/or protonation The inter-woon between water molecules and DNA triplex was visualized in terms,of the end-to-end distance change of each trand The chain length was reduced by about 7 5%-16 2% due to the screening effect In addition,as the screening fleer nerenises,Hoogsteen base pairs appear to be stable in triplex formation.Our computational results also show this the effect of protonated- and methylated cytosme on the stability of triplex formation was due to the change of the beerostanis mteractions rather than the surface stacking interaction</description><subject>analysis</subject><subject>computational</subject><subject>DNA</subject><subject>effect</subject><subject>Oligonucleotides</subject><subject>Protonation</subject><subject>Screening</subject><subject>stability</subject><subject>Stability analysis</subject><subject>triplex</subject><issn>1674-7291</issn><issn>1006-9291</issn><issn>1869-1870</issn><issn>1862-2771</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNpFkE1PwzAMhiMEEtPYhV9QiRtSIF9tEm4wMUCaxAXOVZomW6e26ZJUov-ebEPMB9uyH1v2C8AtRg8YIf74skJE8IIhfAFmWBQSYsHRZcoLziAnEl-DRQg7lIxSRHg-A7ul64Yxqti4XrWZSm4KTciczaJvhtb8ZNb57tg_FF3bbFw_6ta42NQmPA3exTQaTZ2G6yyHnYnbqT0WhgkOIxymrEuwvQFXVrXBLP7iHHyvXr-W73D9-faxfF5DTUQeYW0Z11piRajQRirEJLOaYGGExhppWeWcMYVqI6taVZxzRYqcMlyjguWVoXNwd9qbTtuPJsRy50afHgtlkkAUBZWSJOr-RGnvQvDGloNvOuWnEqPyIGd5lvO8Um9dv9k3_eafpgjhREhG6C8HGnRl</recordid><startdate>19970401</startdate><enddate>19970401</enddate><creator>白春礼 王琛 汪新文</creator><general>Springer Nature B.V</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>~WA</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7XB</scope><scope>88I</scope><scope>8FE</scope><scope>8FG</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>KB.</scope><scope>M2P</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope></search><sort><creationdate>19970401</creationdate><title>Computational analysis of triplex formation of oligonucleotides:protonated and 5-methylated py-pu-py motif</title><author>白春礼 王琛 汪新文</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c285t-df47cc91a238ce9a0494fc218e8c1c0c9b5744a0de9bdab777a265341d0645be3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>analysis</topic><topic>computational</topic><topic>DNA</topic><topic>effect</topic><topic>Oligonucleotides</topic><topic>Protonation</topic><topic>Screening</topic><topic>stability</topic><topic>Stability analysis</topic><topic>triplex</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>白春礼 王琛 汪新文</creatorcontrib><collection>维普_期刊</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Materials Science Database</collection><collection>Science Database</collection><collection>Materials science collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><jtitle>Science China. Chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>白春礼 王琛 汪新文</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Computational analysis of triplex formation of oligonucleotides:protonated and 5-methylated py-pu-py motif</atitle><jtitle>Science China. Chemistry</jtitle><addtitle>SCIENCE CHINA Chemistry</addtitle><date>1997-04-01</date><risdate>1997</risdate><volume>40</volume><issue>2</issue><spage>113</spage><epage>121</epage><pages>113-121</pages><issn>1674-7291</issn><issn>1006-9291</issn><eissn>1869-1870</eissn><eissn>1862-2771</eissn><abstract>A triple helix composed of three dodecanucleotides,5’-d(TC)6,5-(AG)6 and 3’-d(TC)6 with each containing a methylcytosme or a protonated-cylosine,was examined in terms of various interactions Computational malvsis of the stability of triplex formation was consistent with the experimental observations.The Hoogsleen hase among interaction was increased through substitution of 5-methylcytosme for cytosme and/or protonation The inter-woon between water molecules and DNA triplex was visualized in terms,of the end-to-end distance change of each trand The chain length was reduced by about 7 5%-16 2% due to the screening effect In addition,as the screening fleer nerenises,Hoogsteen base pairs appear to be stable in triplex formation.Our computational results also show this the effect of protonated- and methylated cytosme on the stability of triplex formation was due to the change of the beerostanis mteractions rather than the surface stacking interaction</abstract><cop>Heidelberg</cop><pub>Springer Nature B.V</pub><doi>10.1007/BF02876401</doi><tpages>9</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1674-7291
ispartof Science China. Chemistry, 1997-04, Vol.40 (2), p.113-121
issn 1674-7291
1006-9291
1869-1870
1862-2771
language eng
recordid cdi_proquest_journals_2918663992
source Springer Nature
subjects analysis
computational
DNA
effect
Oligonucleotides
Protonation
Screening
stability
Stability analysis
triplex
title Computational analysis of triplex formation of oligonucleotides:protonated and 5-methylated py-pu-py motif
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-18T21%3A29%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Computational%20analysis%20of%20triplex%20formation%20of%20oligonucleotides:protonated%20and%205-methylated%20py-pu-py%20motif&rft.jtitle=Science%20China.%20Chemistry&rft.au=%E7%99%BD%E6%98%A5%E7%A4%BC%20%E7%8E%8B%E7%90%9B%20%E6%B1%AA%E6%96%B0%E6%96%87&rft.date=1997-04-01&rft.volume=40&rft.issue=2&rft.spage=113&rft.epage=121&rft.pages=113-121&rft.issn=1674-7291&rft.eissn=1869-1870&rft_id=info:doi/10.1007/BF02876401&rft_dat=%3Cproquest_cross%3E2918663992%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c285t-df47cc91a238ce9a0494fc218e8c1c0c9b5744a0de9bdab777a265341d0645be3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2918663992&rft_id=info:pmid/&rft_cqvip_id=3001013942&rfr_iscdi=true