Study of the Disorders of the Blood System of Irradiated and Unirradiated Animals Kept in Contact

• Published in: Biology bulletin of the Russian Academy of Sciences 2023-12, Vol.50 (11), p.2956-2961
• Main Authors: Pelevina, I. I., Kogarko, I. N., Pryakhin, E. A., Petushkova, V. V., Kogarko, B. S., Neyfakh, E. A., Andreev, S. S., Ktitorova, O. V., Ganeev, I. I.
• Format: Article
• Language: English
• Subjects: Biochemistry; Biomedical and Life Sciences; Bone marrow; Cell Biology; Ecology; General Radiobiology; Life Sciences; Radiation; Septum; Spleen; Statistical analysis; Thymus; Thymus gland; Zoology
• ISSN: 1062-3590; 1608-3059
• DOI: 10.1134/S1062359023110171

Radiation-induced “bystander effects” were studied at the inter-organismal level. The experiment used mice irradiated at a dose of 3 Gy and nonirradiated bystander mice, kept together for 90 days. An analysis of the state of the bone marrow, spleen, and thymus in the experimental animals was carried out, which included an assessment of the mass of the spleen and thymus, as well as the number of nuclear cells in the bone marrow, spleen, and thymus in mice. The results of this study indicate the presence of a “bystander effect” in the nucleated cells of the bone marrow of mice. In unirradiated animals that were kept with irradiated mice in a cage without a septum, the number of nucleated cells is reduced statistically significantly ( t = 2.58; p = 0.02) compared to the control. There is an effect of increasing the number of nucleated cells in the bone marrow in the group of irradiated mice in a cage with a septum, statistically significant by 15% more than in the gamma control group ( t = 2.36; p = 0.03), i.e., the “rescue effect.” Obviously, in all organs of the hematopoietic system—bone marrow, thymus, and spleen—there are violations associated with the factor of contact of animals. It is possible that the observed radiation-induced “nontargeted effects” are realized at the level of induced changes in the DNA.