Split G-quadruplex based PfAgo sensing platform for nucleotide mutation discrimination and human genotyping

A PfAgo-G4 sensing platform exploiting G4 as a signal reporter was proposed, validated, and optimized. By introducing two mismatches at the Link strand, a universal nucleotide design rule was established for accurate single nucleotide polymorphism discrimination with PfAgo-G4. The FUT2 gene was then...

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Bibliographic Details
Published in:Analyst (London) 2024-01, Vol.149 (3), p.77-711
Main Authors: Zhang, Yan, Gong, Bin, Lin, Yanan, Zhu, Yuedong, Su, Gaoxing, Yu, Yanyan
Format: Article
Language:English
Subjects:
Aptamers, Nucleotide - genetics
G-Quadruplexes
Genotype
Humans
Nucleotides
Polymorphism
Polymorphism, Single Nucleotide
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Summary:A PfAgo-G4 sensing platform exploiting G4 as a signal reporter was proposed, validated, and optimized. By introducing two mismatches at the Link strand, a universal nucleotide design rule was established for accurate single nucleotide polymorphism discrimination with PfAgo-G4. The FUT2 gene was then successfully and accurately genotyped using human buccal swab samples. A split G-quadruplex based PfAgo sensing platform was developed for nucleotide mutation discrimination and human genotyping.
ISSN:0003-2654
1364-5528
DOI:10.1039/d3an02090h