SSCP Analysis of Variations in Haplotypes of Citrus Tristeza Virus Isolated from Yuzu (Citrus junos) in Geographically Separate Regions of Korea

Yuzu (Citrus junos) trees were examined from six geographically separate provinces in the Republic of Korea, including four islands (Geoje, Namhae, Wan, and Jeju), 1 peninsula (Goheung), and 1 inland area (Boseong). The population of sequence variants of citrus tristeza virus (CTV) was isolated and...

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Published in:Journal of plant biology = Singmul Hakhoe chi 2006-02, Vol.49 (1), p.88-96
Main Authors: Kim, D.H. (Rural Development Administration, Suwon, Republic of Korea), Shim, H.K. (Sungkyunkwan University, Suwon, Republic of Korea), Hyeon, J.W. (National Institute of Subtropical Agriculture, RDA, Jeju, Republic of Korea), Kwon, H.M. (National Institute of Subtropical Agriculture, RDA, Jeju, Republic of Korea), Kim, K.S. (National Institute of Subtropical Agriculture, RDA, Jeju, Republic of Korea), Choi, M.S. (Sungkyunkwan University, Suwon, Republic of Korea), Lee, J.K. (Sungkyunkwan University, Suwon, Republic of Korea), Kim, D.G. (Purdue University, IN, USA), Yang, J.S. (Sungkyunkwan University, Suwon, Republic of Korea), E-mail: jsyang@skku.ac.kr, Lee, S.C. (Sungkyunkwan University, Suwon, Republic of Korea), E-mail: sukchan@skku.ac.kr
Format: Article
Language:English
Subjects:
Citrus junos
CITRUS TRISTEZA CLOSTEROVIRUS
Closterovirus
CLOSTEROVIRUS TRISTEZA DEL CITRUS
CLOSTEROVIRUS TRISTEZA DU CITRUS
Conformation
Deoxyribonucleic acid
DNA
Genomes
Haplotypes
Mixed infection
Nucleotide sequence
Nucleotides
p20 gene
Plant viruses
Polymorphism
Signs and symptoms
single-strand conformation polymorphism analysis
Stem pitting
viral RNA population
Viruses
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Summary:Yuzu (Citrus junos) trees were examined from six geographically separate provinces in the Republic of Korea, including four islands (Geoje, Namhae, Wan, and Jeju), 1 peninsula (Goheung), and 1 inland area (Boseong). The population of sequence variants of citrus tristeza virus (CTV) was isolated and analyzed by single-strand conformation polymorphism (SSCP) analysis of cDNA from the p20 gene. SSCP profiles of 65 PCR products showed different band patterns but with similar intensities. Sixteen haplotypes were subgrouped according to their SSCP profiles and severity of symptoms.
ISSN:1226-9239
1867-0725
DOI:10.1007/bf03030792