Microfluidic Potentiometric Cytometry for Size-Selective Micro Dispersion Analysis

Impedance-based microfluidic cytometry has proven its potential for applications in point-of-care tests and clinical diagnosis. In this work, we have developed a three-electrode microfluidic potentiometric cytometry system for robust cell size and concentration measurement. A pair of planar electrod...

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Bibliographic Details
Published in:Biochip journal 2022-12, Vol.16 (4), p.471-479
Main Authors: Kim, Bongjoon, Yao, Wenliang, Rhie, Jong Won, Chun, Honggu
Format: Article
Language:English
Subjects:
Amplitudes
Biomedical Engineering and Bioengineering
Biotechnology
Cell size
Chemistry
Chemistry and Materials Science
Cytometry
Diameters
Dielectric properties
Electric fields
Electrodes
Environmental monitoring
Mathematical models
Microchannels
Microfluidics
Microparticles
Original Article
Particle size
Polystyrene
Polystyrene resins
Public health
Robustness (mathematics)
Signal processing
Simulation
Stem cells
Voltage drop
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Summary:Impedance-based microfluidic cytometry has proven its potential for applications in point-of-care tests and clinical diagnosis. In this work, we have developed a three-electrode microfluidic potentiometric cytometry system for robust cell size and concentration measurement. A pair of planar electrodes applies a constant voltage drop as a sensing electrode detects the potential changes caused by particle translocation events through a microchannel. The microchannel geometry was optimized based on numerical simulation to provide signal amplitudes of particles independent of cross-sectional position as well as buffer conductivity. Additionally, the signal amplitude shows non-linear characteristics with respect to particle diameter, enabling stem cell selective counting without further signal processing. Experimental results from measurements of the concentration of polystyrene particles as well as stem cells in a stromal vascular fraction (SVF) showed good agreement with manual hemocytometry (error 
ISSN:1976-0280
2092-7843
DOI:10.1007/s13206-022-00083-y