Transcript profile of transgenicArabidopsis constitutively producing methyl jasmonate

Using an Affymetrix GeneChip™ containing 8300 oligonucleotide probes, we measured transcript levels in transgenicArabidopsis overexpressing the jasmonate carboxyl methyltransferase gene (AtJMT). When compared with wild-type plants, 5-week-old transgenics exhibited significant alterations (more than...

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Bibliographic Details
Published in:Journal of plant biology = Singmul Hakhoe chi 2007-02, Vol.50 (1), p.12-17
Main Authors: Jung, Choonkyun, Yeu, Song Yion, Koo, Yeon Jong, Kim, Minkyun, Choi, Yang Do, Cheong, Jong- Joo
Format: Article
Language:English
Subjects:
Drought
Gene expression
Genes
Methyl jasmonate
Methyltransferase
Oligonucleotides
Oxidative stress
Photosynthesis
Plants (botany)
Senescence
Transgenic plants
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Summary:Using an Affymetrix GeneChip™ containing 8300 oligonucleotide probes, we measured transcript levels in transgenicArabidopsis overexpressing the jasmonate carboxyl methyltransferase gene (AtJMT). When compared with wild-type plants, 5-week-old transgenics exhibited significant alterations (more than a two-fold increase or decrease) in the expression levels of 168 genes. Among them, 80 were up-regulated, including those involved in defense, oxidative stress-tolerance, and senescence. In contrast, the expression of 88 genes, including those that function in photosynthesis and cold/drought-stress responses, was significantly down-regulated. Thus, endogenous generation of methyl jasmonate through AtjMT-overexpression modified the transcript levels of genes previously identified as being jasmonate-responsive. This result confirms that MeJA formation is a key control point for jasmonate-responsive gene expression in plants.
ISSN:1226-9239
1867-0725
DOI:10.1007/BF03030594