Rapid and cost-effective molecular identification of the three mackerel species of the genus Rastrelliger (Perciformes: Scombridae) using PCR-RFLP analysis

The three mackerel species, Rastrelliger kanagurta , Rastrelliger brachysoma , and Rastrelliger faughni , co-occur in the central part of the Indo-West Pacific Ocean, and together represent important fisheries resources. However, they have sometimes been collectively referred to as “ Rastrelliger sp...

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Bibliographic Details
Published in:Marine biodiversity 2017-06, Vol.47 (2), p.609-611
Main Authors: Muto, Nozomu, Takeshima, Hirohiko, Kakioka, Ryo, Alama, Ulysses B., Guzman, Armi May T., Cruz, Ramon S., Gaje, Arnold C., Traifalgar, Rex Ferdinand M., Motomura, Hiroyuki, Muto, Fumihito, Babaran, Ricardo P., Ishikawa, Satoshi
Format: Article
Language:English
Subjects:
Animal Systematics/Taxonomy/Biogeography
Biodiversity
Biomedical and Life Sciences
Cytochrome
Cytochrome b
Cytochromes
DNA
Enzymes
Fish
Fisheries
Fisheries management
Fishery resources
Fishery statistics
Fishing
Freshwater & Marine Ecology
Identification
Landing statistics
Life Sciences
Mackerel
Marine fishes
Mitochondrial DNA
Morphology
Museums
Nucleotide sequence
PCR
Plant Systematics/Taxonomy/Biogeography
Polymerase chain reaction
Polymorphism
Population status
Rastrelliger
Resource management
Restriction fragment length polymorphism
Short Communication
Species
Statistical analysis
Transfer RNA
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Summary:The three mackerel species, Rastrelliger kanagurta , Rastrelliger brachysoma , and Rastrelliger faughni , co-occur in the central part of the Indo-West Pacific Ocean, and together represent important fisheries resources. However, they have sometimes been collectively referred to as “ Rastrelliger spp.” in the fisheries statistics, owing to their morphological similarity. Therefore, species-specific data on landings, effort, and population status are lacking, undermining the appropriate resource management. The present study provides a rapid and cost-effective method based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis to distinguish the three species. Digestion of the PCR products of 1247 base pairs of mitochondrial DNA encompassing the complete sequence of cytochrome b gene using two restriction enzymes yielded diagnostic restriction patterns for the three species, thereby enabling accurate identification.
ISSN:1867-1616
1867-1624
DOI:10.1007/s12526-016-0537-7