Developing Thick Cardiac Tissue with a Multilayer Fiber Sheet for Treating Myocardial Infarction

Human-induced pluripotent stem cell (hiPSC)-derived cardiac patches have been extensively used for treating myocardial infarction and have shown potential for clinical application. However, the limited patch thickness can hamper its therapeutic effect. We previously developed a fibrous scaffold that...

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Bibliographic Details
Published in:Advanced fiber materials (Online) 2023-12, Vol.5 (6), p.1905-1918
Main Authors: Li, Junjun, Qu, Xiang, Liu, Li, Li, Lingjun, Hua, Ying, Zhang, Jingbo, Ishida, Masako, Yoshida, Noriko, Tabata, Akiko, Sougawa, Nagako, Ito, Emiko, Mochizuki-Oda, Noriko, Harada, Akima, Kawamura, Takuji, Matsuura, Ryohei, Wang, Yingzhe, Morishima, Keisuke, Miyagawa, Shigeru, Sawa, Yoshiki
Format: Article
Language:English
Subjects:
Aluminum
Angiogenesis
Antibodies
Cardiac arrhythmia
Chemistry and Materials Science
Clinical trials
Cytokines
Ejection fraction
Flow cytometry
Flow velocity
Heart attacks
Histology
Human influences
Materials Engineering
Materials Science
Multilayers
Myocardial infarction
Nanoscale Science and Technology
Ostomy
Polymer Sciences
Renewable and Green Energy
Research Article
Scaffolds
Shear stress
Software
Stem cells
Textile Engineering
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Summary:Human-induced pluripotent stem cell (hiPSC)-derived cardiac patches have been extensively used for treating myocardial infarction and have shown potential for clinical application. However, the limited patch thickness can hamper its therapeutic effect. We previously developed a fibrous scaffold that allowed the formation of well-organized cardiac tissue constructs. In the present study, based on the above technology, we developed a three-dimensional multilayer fibrous scaffold with dynamic perfusion, on which approximately 20 million hiPSC-derived cardiomyocytes (CMs) could be seeded in a single step and organized into 1 mm thick and viable tissue. The multilayer cardiac tissue demonstrated enhanced contractile properties and upregulated cytokine secretion compared with the control group. Notably, when used on the myocardial infarction model, the multilayer group showed improved functional recovery and less fibrosis. These results indicated that the appropriate hiPSC-CM dose requires careful evaluation in developing clinical therapy. The multilayer cardiac tissue group demonstrated significant improvement than the control group, indicating that higher doses of transplanted cells may have improved therapeutic effects in treating myocardial infarction.
ISSN:2524-7921
2524-793X
DOI:10.1007/s42765-023-00313-4