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Enhancement of intranasal mucosal immunization of mucosal vaccines by ultrasonic treatment
The pathogens of most infectious diseases invade the host through mucosal sites, and immunization with mucosal vaccines is the best means of combating these infectious diseases. Oral delivery and nasal delivery are the most common methods of mucosal vaccination. However, the delivery process is inef...
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| Published in: | Bio-design and manufacturing 2023-07, Vol.6 (4), p.405-422 |
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| cites | cdi_FETCH-LOGICAL-c396t-97b7cd5ee393d262d44d9778b4878a8148c5dcb56ec8c311499de5445ff228e93 |
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| creator | Xu, Haowei Liao, Yang Svetlana, Mankovskaya Yang, Deguang Wan, Huaibin Liu, Zonghua |
| description | The pathogens of most infectious diseases invade the host through mucosal sites, and immunization with mucosal vaccines is the best means of combating these infectious diseases. Oral delivery and nasal delivery are the most common methods of mucosal vaccination. However, the delivery process is inefficient, and mucosal vaccination is ineffective because the vaccine formulation is easily and rapidly removed and has difficulty in crossing the mucosal surface. In this paper, we investigated whether the mucosal immune response could be enhanced by ultrasound facilitation of nasal mucosal delivery of vaccine preparations. For this purpose, we used manganese dioxide (MnO
2
) as the vaccine carrier/adjuvant, coated with chitosan oligosaccharide (COS) to enhance mucosal adsorption, and further physically adsorbed model antigen ovalbumin (OVA) to construct a nanoparticulate vaccine formulation MnO
2
@COS@OVA. Ultrasound treatment was found to promote antigen delivery and recruitment of dendritic cells (DCs) and macrophages as well as T-cell infiltration in nasal mucosal tissues through nasal mucosal immunization studies. With ultrasound assistance, MnO
2
@COS@OVA particles promoted the maturation of DCs in vitro and in vivo and promoted the production of effector memory T cells in vivo and cytokine secretion by splenocytes in vitro. In particular, ultrasound treatment significantly increased the levels of secretory IgA antibodies in the nasal mucosa and genital tract mucosa of experimental mice. In addition, the experimental data showed that the MnO
2
@COS@OVA particles had good biocompatibility and caused no significant damage to the nasal mucosal and vital organ tissue. These data suggest that ultrasound treatment can promote the induction of efficient immune responses to mucosal vaccines and provide new ideas for the opening and clinical translation of mucosal vaccines.
Graphic abstract |
| doi_str_mv | 10.1007/s42242-023-00231-9 |
| format | article |
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2
) as the vaccine carrier/adjuvant, coated with chitosan oligosaccharide (COS) to enhance mucosal adsorption, and further physically adsorbed model antigen ovalbumin (OVA) to construct a nanoparticulate vaccine formulation MnO
2
@COS@OVA. Ultrasound treatment was found to promote antigen delivery and recruitment of dendritic cells (DCs) and macrophages as well as T-cell infiltration in nasal mucosal tissues through nasal mucosal immunization studies. With ultrasound assistance, MnO
2
@COS@OVA particles promoted the maturation of DCs in vitro and in vivo and promoted the production of effector memory T cells in vivo and cytokine secretion by splenocytes in vitro. In particular, ultrasound treatment significantly increased the levels of secretory IgA antibodies in the nasal mucosa and genital tract mucosa of experimental mice. In addition, the experimental data showed that the MnO
2
@COS@OVA particles had good biocompatibility and caused no significant damage to the nasal mucosal and vital organ tissue. These data suggest that ultrasound treatment can promote the induction of efficient immune responses to mucosal vaccines and provide new ideas for the opening and clinical translation of mucosal vaccines.
Graphic abstract</description><identifier>ISSN: 2096-5524</identifier><identifier>EISSN: 2522-8552</identifier><identifier>DOI: 10.1007/s42242-023-00231-9</identifier><language>eng</language><publisher>Singapore: Springer Nature Singapore</publisher><subject>Antigens ; Biocompatibility ; Biomaterials ; Biomedical Engineering and Bioengineering ; Chitosan ; Dendritic cells ; Effector cells ; Engineering ; Ethanol ; Genital tract ; Immunoglobulin A ; Immunological memory ; Infectious diseases ; Laboratory animals ; Lymphocytes T ; Macrophages ; Manganese ; Manganese dioxide ; Mechanical Engineering ; Medical research ; Memory cells ; Mucosal immunity ; Nanoparticles ; Oligosaccharides ; Ovalbumin ; Pathogens ; Potassium ; Proteins ; Research Article ; Splenocytes ; Ultrasonic imaging ; Ultrasound ; Vaccines</subject><ispartof>Bio-design and manufacturing, 2023-07, Vol.6 (4), p.405-422</ispartof><rights>Zhejiang University Press 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c396t-97b7cd5ee393d262d44d9778b4878a8148c5dcb56ec8c311499de5445ff228e93</citedby><cites>FETCH-LOGICAL-c396t-97b7cd5ee393d262d44d9778b4878a8148c5dcb56ec8c311499de5445ff228e93</cites><orcidid>0000-0002-5840-7191</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Xu, Haowei</creatorcontrib><creatorcontrib>Liao, Yang</creatorcontrib><creatorcontrib>Svetlana, Mankovskaya</creatorcontrib><creatorcontrib>Yang, Deguang</creatorcontrib><creatorcontrib>Wan, Huaibin</creatorcontrib><creatorcontrib>Liu, Zonghua</creatorcontrib><title>Enhancement of intranasal mucosal immunization of mucosal vaccines by ultrasonic treatment</title><title>Bio-design and manufacturing</title><addtitle>Bio-des. Manuf</addtitle><description>The pathogens of most infectious diseases invade the host through mucosal sites, and immunization with mucosal vaccines is the best means of combating these infectious diseases. Oral delivery and nasal delivery are the most common methods of mucosal vaccination. However, the delivery process is inefficient, and mucosal vaccination is ineffective because the vaccine formulation is easily and rapidly removed and has difficulty in crossing the mucosal surface. In this paper, we investigated whether the mucosal immune response could be enhanced by ultrasound facilitation of nasal mucosal delivery of vaccine preparations. For this purpose, we used manganese dioxide (MnO
2
) as the vaccine carrier/adjuvant, coated with chitosan oligosaccharide (COS) to enhance mucosal adsorption, and further physically adsorbed model antigen ovalbumin (OVA) to construct a nanoparticulate vaccine formulation MnO
2
@COS@OVA. Ultrasound treatment was found to promote antigen delivery and recruitment of dendritic cells (DCs) and macrophages as well as T-cell infiltration in nasal mucosal tissues through nasal mucosal immunization studies. With ultrasound assistance, MnO
2
@COS@OVA particles promoted the maturation of DCs in vitro and in vivo and promoted the production of effector memory T cells in vivo and cytokine secretion by splenocytes in vitro. In particular, ultrasound treatment significantly increased the levels of secretory IgA antibodies in the nasal mucosa and genital tract mucosa of experimental mice. In addition, the experimental data showed that the MnO
2
@COS@OVA particles had good biocompatibility and caused no significant damage to the nasal mucosal and vital organ tissue. These data suggest that ultrasound treatment can promote the induction of efficient immune responses to mucosal vaccines and provide new ideas for the opening and clinical translation of mucosal vaccines.
Graphic abstract</description><subject>Antigens</subject><subject>Biocompatibility</subject><subject>Biomaterials</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Chitosan</subject><subject>Dendritic cells</subject><subject>Effector cells</subject><subject>Engineering</subject><subject>Ethanol</subject><subject>Genital tract</subject><subject>Immunoglobulin A</subject><subject>Immunological memory</subject><subject>Infectious diseases</subject><subject>Laboratory animals</subject><subject>Lymphocytes T</subject><subject>Macrophages</subject><subject>Manganese</subject><subject>Manganese dioxide</subject><subject>Mechanical Engineering</subject><subject>Medical research</subject><subject>Memory cells</subject><subject>Mucosal immunity</subject><subject>Nanoparticles</subject><subject>Oligosaccharides</subject><subject>Ovalbumin</subject><subject>Pathogens</subject><subject>Potassium</subject><subject>Proteins</subject><subject>Research Article</subject><subject>Splenocytes</subject><subject>Ultrasonic imaging</subject><subject>Ultrasound</subject><subject>Vaccines</subject><issn>2096-5524</issn><issn>2522-8552</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp9kE9LxDAQxYMouKz7BTwVPEeTSdImR1nWP7DgRS9eQpqmGtmma9IK66c3tYo3L_OG4b3fwEPonJJLSkh1lTgAB0yAYZIHxeoILUAAYCkEHOedqBLnlZ-iVUq-JkwJlZNygZ434dUE6zoXhqJvCx-GaIJJZld0o-0n9V03Bv9pBt-HyfJ7_zDW-uBSUR-KcZdjqQ_eFkN0ZphwZ-ikNbvkVj-6RE83m8f1Hd4-3N6vr7fYMlUOWFV1ZRvhHFOsgRIazhtVVbLmspJGUi6taGwtSmelZZRypRonOBdtCyCdYkt0MXP3sX8fXRr0Wz_GkF9qUEBBSChFdsHssrFPKbpW76PvTDxoSvRUo55r1LlB_V2jntBsDqVsDi8u_qH_SX0ByJ52VA</recordid><startdate>20230701</startdate><enddate>20230701</enddate><creator>Xu, Haowei</creator><creator>Liao, Yang</creator><creator>Svetlana, Mankovskaya</creator><creator>Yang, Deguang</creator><creator>Wan, Huaibin</creator><creator>Liu, Zonghua</creator><general>Springer Nature Singapore</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PSYQQ</scope><orcidid>https://orcid.org/0000-0002-5840-7191</orcidid></search><sort><creationdate>20230701</creationdate><title>Enhancement of intranasal mucosal immunization of mucosal vaccines by ultrasonic treatment</title><author>Xu, Haowei ; Liao, Yang ; Svetlana, Mankovskaya ; Yang, Deguang ; Wan, Huaibin ; Liu, Zonghua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c396t-97b7cd5ee393d262d44d9778b4878a8148c5dcb56ec8c311499de5445ff228e93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Antigens</topic><topic>Biocompatibility</topic><topic>Biomaterials</topic><topic>Biomedical Engineering and Bioengineering</topic><topic>Chitosan</topic><topic>Dendritic cells</topic><topic>Effector cells</topic><topic>Engineering</topic><topic>Ethanol</topic><topic>Genital tract</topic><topic>Immunoglobulin A</topic><topic>Immunological memory</topic><topic>Infectious diseases</topic><topic>Laboratory animals</topic><topic>Lymphocytes T</topic><topic>Macrophages</topic><topic>Manganese</topic><topic>Manganese dioxide</topic><topic>Mechanical Engineering</topic><topic>Medical research</topic><topic>Memory cells</topic><topic>Mucosal immunity</topic><topic>Nanoparticles</topic><topic>Oligosaccharides</topic><topic>Ovalbumin</topic><topic>Pathogens</topic><topic>Potassium</topic><topic>Proteins</topic><topic>Research Article</topic><topic>Splenocytes</topic><topic>Ultrasonic imaging</topic><topic>Ultrasound</topic><topic>Vaccines</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, Haowei</creatorcontrib><creatorcontrib>Liao, Yang</creatorcontrib><creatorcontrib>Svetlana, Mankovskaya</creatorcontrib><creatorcontrib>Yang, Deguang</creatorcontrib><creatorcontrib>Wan, Huaibin</creatorcontrib><creatorcontrib>Liu, Zonghua</creatorcontrib><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest One Psychology</collection><jtitle>Bio-design and manufacturing</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Haowei</au><au>Liao, Yang</au><au>Svetlana, Mankovskaya</au><au>Yang, Deguang</au><au>Wan, Huaibin</au><au>Liu, Zonghua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhancement of intranasal mucosal immunization of mucosal vaccines by ultrasonic treatment</atitle><jtitle>Bio-design and manufacturing</jtitle><stitle>Bio-des. Manuf</stitle><date>2023-07-01</date><risdate>2023</risdate><volume>6</volume><issue>4</issue><spage>405</spage><epage>422</epage><pages>405-422</pages><issn>2096-5524</issn><eissn>2522-8552</eissn><abstract>The pathogens of most infectious diseases invade the host through mucosal sites, and immunization with mucosal vaccines is the best means of combating these infectious diseases. Oral delivery and nasal delivery are the most common methods of mucosal vaccination. However, the delivery process is inefficient, and mucosal vaccination is ineffective because the vaccine formulation is easily and rapidly removed and has difficulty in crossing the mucosal surface. In this paper, we investigated whether the mucosal immune response could be enhanced by ultrasound facilitation of nasal mucosal delivery of vaccine preparations. For this purpose, we used manganese dioxide (MnO
2
) as the vaccine carrier/adjuvant, coated with chitosan oligosaccharide (COS) to enhance mucosal adsorption, and further physically adsorbed model antigen ovalbumin (OVA) to construct a nanoparticulate vaccine formulation MnO
2
@COS@OVA. Ultrasound treatment was found to promote antigen delivery and recruitment of dendritic cells (DCs) and macrophages as well as T-cell infiltration in nasal mucosal tissues through nasal mucosal immunization studies. With ultrasound assistance, MnO
2
@COS@OVA particles promoted the maturation of DCs in vitro and in vivo and promoted the production of effector memory T cells in vivo and cytokine secretion by splenocytes in vitro. In particular, ultrasound treatment significantly increased the levels of secretory IgA antibodies in the nasal mucosa and genital tract mucosa of experimental mice. In addition, the experimental data showed that the MnO
2
@COS@OVA particles had good biocompatibility and caused no significant damage to the nasal mucosal and vital organ tissue. These data suggest that ultrasound treatment can promote the induction of efficient immune responses to mucosal vaccines and provide new ideas for the opening and clinical translation of mucosal vaccines.
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| ispartof | Bio-design and manufacturing, 2023-07, Vol.6 (4), p.405-422 |
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| source | Springer Nature |
| subjects | Antigens Biocompatibility Biomaterials Biomedical Engineering and Bioengineering Chitosan Dendritic cells Effector cells Engineering Ethanol Genital tract Immunoglobulin A Immunological memory Infectious diseases Laboratory animals Lymphocytes T Macrophages Manganese Manganese dioxide Mechanical Engineering Medical research Memory cells Mucosal immunity Nanoparticles Oligosaccharides Ovalbumin Pathogens Potassium Proteins Research Article Splenocytes Ultrasonic imaging Ultrasound Vaccines |
| title | Enhancement of intranasal mucosal immunization of mucosal vaccines by ultrasonic treatment |
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